Sprouting and regeneration after pyramidotomy and blockade of the myelin-associated neurite growth inhibitors NI 35/250 in adult rats
Sprouting and regeneration after pyramidotomy and blockade of the myelin-associated neurite growth inhibitors NI 35/250 in adult rats
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DOI：
10.1046/j.1460-9568.1999.00560.x
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发表时间：
1999-04-01
影响因子：
3.4
通讯作者：
Schwab, ME
中科院分区：
文献类型：
--
作者：
Raineteau, O;Z'Graggen, WJ;Schwab, ME
选择性单侧病变皮质脊髓束（CST）在脑干水平（脊髓切断术）和中和髓鞘相关的神经突起生长抑制剂NI-35/250与单克隆抗体（mAb）IN-1后，我们以前观察到一个强大的行为恢复并行增强的结构可塑性的损伤以及未损伤的CST。本研究的重点是在这些成年大鼠的损伤部位切断CST轴突的再生反应。结果显示通过用mAb IN-1处理强烈增强CST纤维的再生发芽。这些芽通过锥体交叉并进入颈脊髓的成功伸长也依赖于这种抗体的存在。在脊髓中，再生纤维很少发现在前CST的位置，大多数的纤维似乎随机分布在整个脊髓的横向范围。
After a selective unilateral lesion of the corticospinal tract (CST) at the level of the brainstem (pyramidotomy) and neutralization of the myelin associated neurite growth inhibitors NI-35/250 with the monoclonal antibody (mAb) IN-1, we had previously observed a strong behavioural recovery in parallel with an enhanced structural plasticity of the lesioned as well as the unlesioned CST. The present study focuses on the regenerative response of the cut CST axons at the lesion site in these adult rats. The results show a strong enhancement of regenerative sprouting of CST fibres by treatment with the mAb IN-1. Successful elongation of these sprouts through the pyramidal decussation and into the cervical spinal cord was also dependent on the presence of this antibody. In the spinal cord, regenerating fibres were rarely found in the position of the former CST; most of the fibres were distributed seemingly randomly over the entire lateral extent of the spinal cord.