Purification of Native or Recombinant ADAMTS2, and Procollagen I Cleavage Assay.
Purification of Native or Recombinant ADAMTS2, and Procollagen I Cleavage Assay.
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DOI：
10.1007/978-1-4939-9698-8_5
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发表时间：
2020
影响因子：
--
通讯作者：
A. Colige
中科院分区：
文献类型：
--
作者：
A. Colige
ADAMTS 构成了一个由 19 种分泌型金属蛋白酶组成的家族，涉及不同的病理生理学条件。它们的大部分作用首先来自对人类和动物自发突变或基因工程动物的分析。然而，所涉及的机制及其全部功能仍然在很大程度上未被认识，部分原因是它们难以生产和纯化为重组活性酶。在这里，我们专门描述与 ADAMTS2、3 和 14 有关的协议、提示和技巧，但仍然与其他 ADAMTS 相关。
ADAMTS constitute a family of 19 secreted metalloproteinases involved in diverse physiopathological conditions. Most of their roles first emerged from analysis of spontaneous human and animal mutations or genetically engineered animals. However, the involved mechanisms and the full repertoire of their functions are still largely unrecognized, in part because they are difficult to produce and purify as recombinant active enzymes. Here we describe protocols, tips, and tricks specifically regarding ADAMTS2, 3, and 14 but still relevant for other ADAMTS.