Organization of cortical microtubules at the plasma membrane in Arabidopsis
Organization of cortical microtubules at the plasma membrane in Arabidopsis
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DOI：
10.1007/s004250050064
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发表时间：
1997-03-01
期刊：
影响因子：
4.3
通讯作者：
Sung, ZR
中科院分区：
文献类型：
--
作者：
McClinton, RS;Sung, ZR
为了了解微管在细胞伸长调控中的作用，我们对拟南芥（Arabidopsis thaliana（L.））细胞形状突变体fass的微管模式进行了表征。嘿通过免疫细胞化学检查微管模式，我们发现fass细胞能够将微管组织成有丝分裂纺锤体和成膜体。在间期或前期。透射电镜证实Fass细胞具有皮质微管。但这些微管没有组织成皮质阵列或前前期带。以染色质凝聚和微管蛋白定位于核膜上作为前前期阶段的标记，我们发现fass细胞虽然缺乏前前期带和皮质排列，但其细胞分裂周期似乎正常。为了查明fass细胞中的缺陷，我们描绘了拟南芥细胞中导致皮质阵列形成的顺序事件，发现fass细胞以与野生型细胞相同的方式启动和激活皮质微管，但未能将它们排列成皮质阵列。总之，这些结果表明，fass细胞受损的微管组织中心（S）的一个组成部分所需的皮层微管在质膜上的正确排序。
To understand the role of microtubules in the regulation of cell elongation, we characterized microtubule patterns in fass, a cell shape mutant of Arabidopsis thaliana (L.) Heynh. Examining microtubule patterns via immunocytochemistry, we found that fass cells were able to organize their microtubules into mitotic spindles and phragmoplasts. During interphase or preprophase. fass cells had cortical microtubules, verified by transmission electron microscopy. but these microtubules were not organized into the cortical array or preprophase band. Using chromatin condensation and tubulin localization on the nuclear envelope as preprophase stage markers, we found that although fass cells lacked the preprophase band and cortical array, their cell division cycle appeared normal. To pinpoint the defect in fass cells, we delineated the sequential events leading to cortical array formation in Arabidopsis cells and found that fass cells initiated and recolonized cortical microtubules in the same manner as wild-type cells, but failed to order them into the cortical array. Taken together, these results suggest fass cells are impaired in a component of the microtubule organizing center(s) required for the proper ordering of cortical microtubules at the plasma membrane.