A CALCIUM-DEPENDENT TRANSIENT OUTWARD CURRENT IN XENOPUS-LAEVIS OOCYTES
A CALCIUM-DEPENDENT TRANSIENT OUTWARD CURRENT IN XENOPUS-LAEVIS OOCYTES
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DOI：
10.1098/rspb.1982.0056
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发表时间：
1982-01-01
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影响因子：
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通讯作者：
MILEDI, R
中科院分区：
文献类型：
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作者：
MILEDI, R
在X.在电压钳下观察左心室卵母细胞。去极化脉冲，从约-100 mV的保持电位给出，当膜电位变得比约-20 mV更正时，引起瞬时外向电流。随着电位逐渐增大，瞬时外向电流先增大后减小。瞬时电流的幅度增加时，外部Ca 2+浓度升高，电流被取消由Mn 2+。显然，当膜去极化时，Ca 2+离子进入卵母细胞并触发外向电流，可能是通过打开Cl-通道。
Membrane currents were investigated in X. laevis oocytes under voltage clamp. Depolarizing pulses, given from a holding potential of about -100 mV, elicited a transient outward current when the membrane potential was made more positive than about -20 mV. As the potential was made increasingly positive the transient outward current first increased and then decreased. The amplitude of the transient current increased when the external Ca2+ concentration was raised; the current was abolished by Mn2+. Apparently when the membrane is depolarized, Ca2+ ions enter the oocyte and trigger an outward current, possibly by opening Cl- channels.