Histological evaluation and Prdm9 expression level in the testis of sterile male cattle-yaks
Histological evaluation and Prdm9 expression level in the testis of sterile male cattle-yaks
复制标题
不育雄性牦牛睾丸组织学评价及Prdm9表达水平
DOI：
10.1016/j.livsci.2013.12.017
复制
发表时间：
2014-02-01
影响因子：
1.8
通讯作者：
Zheng, Y. C.
中科院分区：
文献类型：
--
作者：
Lou, Y. N.;Liu, W. J.;Zheng, Y. C.
本研究的主要目的是揭示杂种不育牦牛（即牛-牦牛）睾丸组织结构和Prdm 9（PR domain containing 9）mRNA水平是否与正常成年牦牛（Bos grunniens）存在差异。利用RT-PCR技术首次从牦牛睾丸组织中克隆到牦牛Prdm 9基因的部分保守序列，得到一个1084 bp的片段，该片段与黄牛Prdm 9基因的核苷酸和氨基酸序列高度相似。基于此序列，建立了Prdm 9基因在成年牦牛（n=8）、牦牛犊牛（n=4）和杂交不育牦牛（n=4）睾丸中的实时定量PCR方法。结果表明，与正常成年牦牛相比，性未成熟牦牛犊和不育公牦牛睾丸中Prdm 9的mRNA水平显著降低。此外，与正常成年牦牛相比，大部分牦牛曲细精管只有支持细胞和少量精原细胞，而没有精母细胞，少数牦牛曲细精管含有粗线期停滞的精母细胞。细胞凋亡分析表明，牦牛精原细胞和（或）精母细胞的凋亡率高于成年牦牛。本研究结果提示Prdm 9基因可能与牦牛雄性不育有关。(C)2013 Elsevier B. V.保留所有权利。
The main objective of the present study was to reveal whether the histological structure of testis and the Prdm9 (PR domain containing 9) mRNA level in testis of hybrid sterile yak (i.e. cattle-yaks) differed from that of normal adult yak (Bos grunniens). A partial conserved sequences of yak Prdm9 gene from yak testis was cloned using RT-PCR for the first time, and a 1084 bp fragment was obtained, which showed high nucleotide and amino acid similarities with those of cattle. Based on this sequence, a quantitative real-time PCR method was developed to compare Prdm9 mRNA levels in the testes of adult yaks (n=8), yak calves (n=4), and hybrid sterile yaks (n=4). Results showed that the mRNA levels of Prdm9 decreased dramatically in the testes of sexually immature yak calves and sterile male cattle-yaks compared with that of normal adult yaks. In addition, compared with normal adult yaks, most seminiferous tubules of cattle-yaks had only Sertoli cells and a few spermatogonia but no spermatocytes, while a few other seminiferous tubules of cattle-yaks contained pachytene arrested spermatocytes. Cell apoptosis analysis showed that more spermatogonial cells and/or spermatocytes in cattle-yaks were subject to apoptosis than adult yaks. Our results suggest that Prdm9 gene might be associated with the male infertility of cattle-yaks. (C) 2013 Elsevier B.V. All rights reserved.