Retrograde tracing with recombinant rabies virus reveals correlations between projection targets and dendritic architecture in layer 5 of mouse barrel cortex.
Retrograde tracing with recombinant rabies virus reveals correlations between projection targets and dendritic architecture in layer 5 of mouse barrel cortex.
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DOI：
10.3389/neuro.04.005.2007
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发表时间：
2007
影响因子：
3.5
通讯作者：
Callaway EM
中科院分区：
文献类型：
--
作者：
Larsen DD;Wickersham IR;Callaway EM
重组狂犬病病毒被用作逆行示踪剂，以允许完全填充的轴突和树突的乔木确定的投射神经元在第5层的小鼠初级体感皮层（S1）在体内。以前的研究已经区分了三种类型的第5层金字塔在S1：tall-tufted，tall-simple和short。第5层锥体神经元逆行标记从几个已知的目标：对侧S1，上级丘，丘脑。重建标记细胞的完整树突状动脉，以明确分类细胞类型。如前所述，我们证实了高簇状锥体投射到上级丘和丘脑，而短的第5层锥体神经元投射到对侧皮质。我们发现，高简单锥体神经元有助于皮质皮质连接。轴突重建显示，皮质投射神经元有一个大的表面轴突树枝化局部，而皮质下投射神经元限制轴突乔木的深层。此外，局部轴突的重建表明，高简单的细胞轴突有广泛的横向扩展，而那些短金字塔更柱状。这些差异是通过在体内完全标记树突和轴突的能力揭示的，并且在以前的研究中使用脑切片中的标记并不明显。
A recombinant rabies virus was used as a retrograde tracer to allow complete filling of the axonal and dendritic arbors of identified projection neurons in layer 5 of mouse primary somatosensory cortex (S1) in vivo. Previous studies have distinguished three types of layer 5 pyramids in S1: tall-tufted, tall-simple, and short. Layer 5 pyramidal neurons were retrogradely labeled from several known targets: contralateral S1, superior colliculus, and thalamus. The complete dendritic arbors of labeled cells were reconstructed to allow for unambiguous classification of cell type. We confirmed that the tall-tufted pyramids project to the superior colliculus and thalamus and that short layer 5 pyramidal neurons project to contralateral cortex, as previously described. We found that tall-simple pyramidal neurons contribute to corticocortical connections. Axonal reconstructions show that corticocortical projection neurons have a large superficial axonal arborization locally, while the subcortically projecting neurons limit axonal arbors to the deep layers. Furthermore, reconstructions of local axons suggest that tall-simple cell axons have extensive lateral spread while those of the short pyramids are more columnar. These differences were revealed by the ability to completely label dendritic and axonal arbors in vivo and have not been apparent in previous studies using labeling in brain slices.