New targets for the cyclic AMP receptor protein in the Escherichia coli K-12 genome
New targets for the cyclic AMP receptor protein in the Escherichia coli K-12 genome
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DOI：
10.1111/j.1574-6968.2007.00826.x
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发表时间：
2007-09-01
影响因子：
2.1
通讯作者：
Lloyd, Georgina S.
中科院分区：
文献类型：
--
作者：
Hollands, Kerry;Busby, Stephen J. W.;Lloyd, Georgina S.
最近对大肠杆菌K-12的基因组研究表明，环腺苷酸受体蛋白（CRP）全局转录调节因子存在大量以前未探索的靶点。这些位点中的11个被克隆，CRP结合在这些目标中的8个被证明。结果表明，CRP可以激活这些目标和CRP的功能性DNA位点的转录。据报道，CRP在aer启动子处起I类激活剂的作用，在gatY、sdaC、ychH和malX启动子处起II类激活剂的作用。
Recent genomic studies with Escherichia coli K-12 have suggested scores of previously unexplored targets for the cyclic AMP receptor protein (CRP) global transcription regulator. Eleven of these loci were cloned and CRP binding was demonstrated at eight of these targets. It is shown that CRP can activate transcription at five of these targets and the functional DNA sites for CRP are identified. It is reported that CRP functions as a Class I activator at the aer promoter and as a Class II activator at the gatY, sdaC, ychH and malX promoters.