Effects of glucose on lactose synthesis in mammary epithelial cells from dairy cow.
Effects of glucose on lactose synthesis in mammary epithelial cells from dairy cow.
复制标题
葡萄糖对奶牛乳腺上皮细胞乳糖合成的影响
DOI：
10.1186/s12917-016-0704-x
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发表时间：
2016-05-26
影响因子：
2.6
通讯作者：
Li Q
中科院分区：
文献类型：
--
作者：
Lin Y;Sun X;Hou X;Qu B;Gao X;Li Q
乳糖作为乳中的主要渗透组分，是乳量的主要决定因素。葡萄糖是乳糖的主要前体。然而，葡萄糖对奶牛乳腺中乳糖合成的影响以及控制这一过程的机制知之甚少。在这里，我们发现葡萄糖有能力诱导奶牛乳腺上皮细胞乳糖合成，以及增加细胞活力和增殖。12 mM葡萄糖的浓度是诱导培养的奶牛乳腺上皮细胞中细胞生长和乳糖合成的最佳浓度。在体外，12 mM葡萄糖增加乳糖含量，同时沿着参与葡萄糖转运和乳糖生物合成途径的基因表达，包括GLUT 1、SLC 35 A2、SLC 35 B1、HK 2、β 4 GalT-I和AKT 1。此外，我们发现AKT 1敲低抑制细胞生长和乳糖合成以及GLUT 1、SLC 35 A2、SLC 35 B1、HK 2和β4GalT-I的表达。葡萄糖诱导奶牛乳腺上皮细胞生长和乳糖合成。蛋白激酶B α作为奶牛乳腺代谢的调节因子，介导葡萄糖对乳糖合成的影响。本文的在线版本（doi：10.1186/s12917-016-0704-x）包含补充材料，可供授权用户使用。
Lactose, as the primary osmotic component in milk, is the major determinant of milk volume. Glucose is the primary precursor of lactose. However, the effect of glucose on lactose synthesis in dairy cow mammary glands and the mechanism governing this process are poorly understood. Here we showed that glucose has the ability to induce lactose synthesis in dairy cow mammary epithelial cells, as well as increase cell viability and proliferation. A concentration of 12 mM glucose was the optimum concentration to induce cell growth and lactose synthesis in cultured dairy cow mammary epithelial cells. In vitro, 12 mM glucose enhanced lactose content, along with the expression of genes involved in glucose transportation and the lactose biosynthesis pathway, including GLUT1, SLC35A2, SLC35B1, HK2, β4GalT-I, and AKT1. In addition, we found that AKT1 knockdown inhibited cell growth and lactose synthesis as well as expression of GLUT1, SLC35A2, SLC35B1, HK2, and β4GalT-I. Glucose induces cell growth and lactose synthesis in dairy cow mammary epithelial cells. Protein kinase B alpha acts as a regulator of metabolism in dairy cow mammary gland to mediate the effects of glucose on lactose synthesis. The online version of this article (doi:10.1186/s12917-016-0704-x) contains supplementary material, which is available to authorized users.