Analysis of leaves from open field-grown transgenic poplars overexpressing xyloglucanase
Analysis of leaves from open field-grown transgenic poplars overexpressing xyloglucanase
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过度表达木葡聚糖酶的露地种植转基因杨树叶的分析
DOI：
10.1007/s10086-011-1247-1
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Hayashi T
中科院分区：
文献类型：
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作者：
Kaku T;Baba K;Taniguchi T;Kurita M;Konagaya K;Ishii K;Kondo T;Serada S;Iizuka H;Kaida R;Taji T;Sakata Y;Hayashi T
研究了35 S启动子介导的杨树木葡聚糖酶cDNA（AaXEG 2）在杨树叶片中的转基因表达。与野生型杨树相比，转基因杨树中的木葡聚糖水平在非肥沃土壤（林地土壤）中降低至15-16%，在肥沃土壤（农田土壤）中降低至21-22%。与野生型植物相比，这些叶子表现出更小的表面积和更圆的牙齿，类似于在孵化室中生长并随后进行温室饲养的太阳叶品种。转基因杨树叶片中多数具有导水维管束的总脉短于野生型。这种脉长的减少可能是由于叶发育期间木葡聚糖的减少，通过蛋白质组学分析，转基因植物的叶中大量蛋白质由此显著下调。转基因杨树的叶子似乎放松了牙齿的边缘，而不是延长了它们的静脉，这是叶子中木葡聚糖纤维素网络松弛的结果。
The transgenic expression ofAspergillusxyloglucanase cDNA (AaXEG2) with 35S promoter in the leaves of open field-grown poplars was studied. The level of xyloglucan in the transgenic poplars was decreased to 15–16% in the non-fertile soil (forest-field soil) and to 21–22% in the fertile soil (farming-field soil) compared with that of the wild-type poplars. The leaves exhibited a smaller surface area with more rounded teeth than those of the wild-type plants, similar to the sun leaf variety that was grown in the incubation room and subsequently greenhoused. The majority of total veins with water-conducting vascular bundles were shorter in the leaves of the transgenic poplars than those of the wild type. This decrease in vein length may result from a decrease in xyloglucan during leaf development, from which large numbers of proteins were markedly downregulated in the leaves of the transgenic plants via proteomic analysis. It seems likely that the leaves of the transgenic poplars came to relax the edges of their tooth rather than extend their veins as a result of the loosening of the xyloglucan cellulose networks in the leaves.