Detection and phylogenetic analysis of Coccidioides posadasii in Arizona soil samples
Detection and phylogenetic analysis of Coccidioides posadasii in Arizona soil samples
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DOI：
10.1016/j.funeco.2011.07.010
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发表时间：
2012-04-01
期刊：
影响因子：
2.9
通讯作者：
Orbach, Marc J.
中科院分区：
文献类型：
--
作者：
Barker, Bridget M.;Tabor, Joseph A.;Orbach, Marc J.
美国西南部球孢子菌病的报告呈上升趋势。然而，球孢子菌这种病原体的生态学仍然不清楚，而且对疾病暴发的环境因素的了解也有限。环境中真菌的检测仍然是疾病来源建模的一个关键挑战。使用 BALB/c 小鼠作为生物传感器，对图森地区（亚利桑那州皮马县）的土壤进行分析，发现 8.9% 的土壤含有病原体。从 11 个土壤中回收的 66 个球孢子菌菌株的基因型通过诊断微卫星位点进行测定。将这些基因型与临床分离株进行比较显示，所有这些都是 Coccidioides posadasii，并且它们与亚利桑那分离株分组。在发现多个菌株的地点中，有两个表明是克隆群体，而其他菌株则产生了多种基因型。这项研究的第二个目标是评估 PCR 的适用性及其高通量筛选的潜力，作为识别含有球孢子菌的土壤的方法。 (C) 2011 Elsevier Ltd 和英国真菌学会。版权所有。
Reports of coccidioidomycosis are on the rise in the southwestern US. However, the ecology of the pathogen, Coccidioides, remains obscure and there is limited knowledge of the environmental antecedents of disease outbreaks. Detection of the fungus in the environment remains a critical challenge to modeling the source of disease. Using BALB/c mice as a biosensor, 8.9% of soils analyzed from the Tucson area (Pima County, Arizona) were found to contain the pathogen. The genotypes of 66 Coccidioides strains, recovered from 11 soils, were determined with diagnostic microsatellite loci. Comparison of these genotypes to clinical isolates revealed all were Coccidioides posadasii and they grouped with Arizona isolates. Among sites where multiple strains were recovered, two indicated a clonal population, while others yielded a diversity of genotypes. A secondary goal of this research was to assess applicability of PCR, with its potential for high-throughput screening, as a method for identifying Coccidioides-containing soils. (C) 2011 Elsevier Ltd and The British Mycological Society. All rights reserved.