Immunohistochemical localization of fibroblast growth factors FGF-1 and FGF-2, and receptors FGFR2 and FGFR3 in the epithelium of human odontogenic cysts and tumors
Immunohistochemical localization of fibroblast growth factors FGF-1 and FGF-2, and receptors FGFR2 and FGFR3 in the epithelium of human odontogenic cysts and tumors
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DOI：
10.1034/j.1600-0714.2001.300708.x
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发表时间：
2001-08-01
影响因子：
3.3
通讯作者：
Wysocki, GP
中科院分区：
文献类型：
--
作者：
So, F;Daley, TD;Wysocki, GP
酸性（FGF-1）和碱性（FGF-2）成纤维细胞生长因子是在多种组织的生长、分化和再生中起作用的生长因子家族的成员。它们在人类牙源性囊肿和肿瘤中的存在以前没有研究过。本研究的目的是检测这些因子和两个成纤维细胞生长因子受体（FGFR 2和FGFR 3）在牙源性囊肿和肿瘤的横截面中的存在，以确定它们是否可能参与牙源性上皮的分化，或者更具体地说，在特定的囊肿或肿瘤的发展。使用存档福尔马林固定石蜡包埋组织。除了一些例外，FGF-2和受体FGFR 2，发现在细胞质中，偶尔在细胞核中的牙源性上皮细胞，而FGF-1和受体FGFR 3，不存在或仅局灶性或微弱检测，使用标准的免疫组织化学技术。这些数据与正常鼠牙发生的数据相似，表明这些因素与牙发生分化有关，而不是发病机制。在成釉细胞纤维瘤和成釉细胞纤维牙瘤中与胚胎间充质相关的牙源性上皮细胞中存在显著的核染色，这表明FGF-2可能参与指导牙源性组织分化阶段的核活性。
Acidic (FGF-1) and basic (FGF-2) fibroblast growth factors are members of a family of growth factors that function in growth, differentiation and regeneration of a variety of tissues. Their presence in human odontogenic cysts and tumors has not been previously investigated. This study was designed to detect immunohistochemically the presence of these factors and two fibroblast growth factor receptors (FGFR2 and FGFR3) in a cross section of odontogenic cysts and tumors, to determine if they may be involved in the differentiation of odontogenic epithelium or, more specifically, in the development of particular cysts or tumors. Archival formalin fixed paraffin-embedded tissues were used. With some exceptions, FGF-2 and the receptor FGFR2, were found in the cytoplasm and occasionally in the nuclei of cells of odontogenic epithelium, while FGF-1 and the receptor FGFR3, were absent or only focally or weakly detected, using standard immunohistochemical techniques. The data are similar to those published for normal murine odontogenesis, suggesting that these factors are associated with odontogenic differentiation rather than pathogenesis. The presence of significant nuclear staining in odontogenic epithelium associated with embryonic mesenchyme in ameloblastic fibromas and ameloblastic fibro-odontomas suggests that FGF-2 may be involved in directing nuclear activity at the histodifferentiation stage of odontogenesis.