CHICKEN HEMATOPOIETIC-CELLS TRANSFORMED BY 7 STRAINS OF DEFECTIVE AVIAN LEUKEMIA VIRUSES DISPLAY 3 DISTINCT PHENOTYPES OF DIFFERENTIATION
CHICKEN HEMATOPOIETIC-CELLS TRANSFORMED BY 7 STRAINS OF DEFECTIVE AVIAN LEUKEMIA VIRUSES DISPLAY 3 DISTINCT PHENOTYPES OF DIFFERENTIATION
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DOI：
10.1016/0092-8674(79)90057-6
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发表时间：
1979-01-01
期刊：
影响因子：
64.5
通讯作者：
GRAF, T
中科院分区：
文献类型：
--
作者：
BEUG, H;VONKIRCHBACH, A;GRAF, T
用7株复制缺陷型禽白血病病毒体外和体内转化鸡造血细胞，测定其6个红系和5个髓系分化参数的表达，包括用新研制的抗血清检测的分化特异性表面抗原。转化细胞表现出3种不同的分化表型。由AEV [禽成红细胞增多症病毒]转化的细胞类似于成红细胞。它们低水平表达血红素、球蛋白、碳酸酐酶和红细胞表面抗原，高水平表达组蛋白H5和成红细胞表面抗原。由MC29、CMII、OK10和MH2病毒转化的细胞具有巨噬细胞样性质。它们强烈表达Fc受体、吞噬能力和巨噬细胞表面抗原，但仅弱表达成髓细胞表面抗原，且ATP酶活性阴性。由AMV [禽成髓细胞瘤病毒]和E26病毒转化的细胞类似于成髓细胞，因为它们弱表达Fc受体、吞噬能力和巨噬细胞表面抗原，但强表达成髓细胞表面抗原和ATP酶活性。在体外和体内转化的细胞之间没有发现差异的参数测试。最近的遗传学和生物化学证据表明，这些表型反映了3种新型病毒转化基因的作用，命名为erb（成红细胞），mac（巨噬细胞）和myb（成髓细胞）。
Chicken hematopoietic cells transformed in vitro and in vivo by 7 strains of replication-defective avian leukemia viruses were assayed for the expression of 6 erythroid and 5 myeloid differentiation parameters, including differentiation-specific surface antigens as detected by newly developed antisera. The transformed cells displayed 3 distinct phenotypes of differentiation. Cells transformed by AEV [avian erythroblastosis virus] resemble erythroblasts. They express heme, globin, carbonic anhydrase and erythrocyte cell surface antigen at low levels, and histone H5 and erythroblast cell surface antigen at high levels. Cells transformed by MC29, CMII, OK10 and MH2 viruses have macrophage-like properties. They strongly express Fc receptors, phagocytic capacity and macrophage cell surface antigen, but only weakly express myeloblast cell surface antigen and are negative for ATPase activity. Cells transformed by AMV [avian myeloblastosis virus] and E26 viruses resemble myeloblasts in that they weakly express Fc receptors, phagocytic capacity and macrophage cell surface antigen but strongly express myeloblast cell surface antigen and ATPase activity. No difference was found between in vitro- and in vivo-transformed cells in the parameters tested. Recent genetic and biochemical evidence showed that these phenotypes reflect the action of 3 new types of viral-transforming genes, designated erb (erythroblast), mac (macrophage) and myb (myeloblast).