Distribution of lectin binding sites in the oviducts of cycling and hormone-treated pigs.
Distribution of lectin binding sites in the oviducts of cycling and hormone-treated pigs.
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循环和激素处理猪输卵管中凝集素结合位点的分布。
DOI：
10.1002/jez.1402650608
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Buhi,WC
中科院分区：
文献类型：
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作者：
Raychoudhury,SS;Suarez,SS;Buhi,WC
配子运输、受精和早期胚胎发育发生在输卵管的不同区域和不同的激素条件下。本研究的目的是使用凝集素来检测影响这些事件的上皮表面糖基化分子分布的变化。在发情周期的第 1 天（发情期）和第 16 天（发情间期）从后备母猪中收集输卵管，并从发情周期第 4 天切除双侧卵巢的后备母猪中收集输卵管，随后用戊酸雌二醇（100 μg/天）、黄体酮（200 mg/天）或玉米油媒介物处理 11 天。六种生物素化凝集素：小麦 (WGA)、花生 (PNA)、欧洲麦芽 (UEA-I)、白豆 (DBA)、蓖麻 (RCA-I) 和刀豆 (Con A)，使用过氧化物酶共轭亲和素作为探针来探测峡部和壶腹部组织。 标记。在周期后备母猪中，在两个周期日，WGA 和 DBA 在峡部的染色比壶腹部的染色更强烈。 PNA 染色呈斑片状，但第 16 天峡部上皮顶端表面的染色比第 1 天更明显。使用 UEA-I，第 16 天壶腹部的染色比第 1 天更强烈。雌二醇治疗后壶腹和峡部的 PNA 和 UEA-I 染色均受到抑制。 DBA staining was suppressed in the isthmus with both estradiol and progesterone treatments.纤毛被除 WGA 和 PNA 之外的所有凝集素标记。因此，猪输卵管中的糖基化模式随着区域和激素状态的不同而变化，反映了可能受到影响的事件的多样性。 © 1993 Wiley-Liss, Inc.
Gamete transport, fertilization, and early embryonic development take place in different regions of the oviduct and under different hormonal conditions. The objective of this study was to use lectins to detect variation in the distribution of glycosylated molecules on the surface of the epithelia that influence these events. Oviducts were collected from gilts on day 1 (estrus) and day 16 (diestrus) of the estrous cycle, and from gilts that were bilaterally ovariectomized on day 4 of the estrous cycle, and were subsequently treated with estradiol valerate (100 μg/day), progesterone (200 mg/day), or corn oil vehicle for 11 days. Six biotinylated lectins,Triticum vulgaris(WGA),Arachis hypogaea(PNA),Ulex europeus(UEA‐I),Dolichos biflorus(DBA),Ricinus communis(RCA‐I), andCanavalia ensiformis(Con A), were used to probe tissue from the isthmus and ampulla using peroxidase‐conjugated avidin as the marker. In cyclic gilts, WGA and DBA stained more strongly in the isthmus than the ampulla on both cycle days. Staining with PNA was patchy, but greater on the apical surface of isthmic epithelium on day 16 than day 1. With UEA‐I, staining was more intense in the ampulla on day 16 than day 1. Staining by PNA and UEA‐I was suppressed in both the ampulla and isthmus with estradiol treatment. DBA staining was suppressed in the isthmus with both estradiol and progesterone treatments. Cilia were labelled by all lectins except WGA and PNA. Thus glycosylation patterns vary in the porcine oviduct with region and hormonal state, reflecting the variety of events that may be influenced. © 1993 Wiley‐Liss, Inc.