Femtosecond cellular transfection using a nondiffracting light beam
Femtosecond cellular transfection using a nondiffracting light beam
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DOI：
10.1063/1.2766835
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发表时间：
2007-07-30
影响因子：
4
通讯作者：
Dholakia, K.
中科院分区：
文献类型：
--
作者：
Tsampoula, X.;Garces-Chavez, V.;Dholakia, K.
选择性渗透细胞膜并引入治疗剂的能力是细胞生物学中的关键目标。光学转染是一种强大的方法，但由于所需的双光子功率密度，需要精确聚焦。作者使用贝塞尔光束，避免了需要精确定位细胞膜，允许双光子激发沿着一条线导致细胞转染。假设最小效率为20%，贝塞尔光束提供的轴向距离比其高斯等效物大20倍的转染。此外，作者证明了由于贝塞尔光束的自愈性质，细胞转染超越了障碍。(C)2007年，美国物理学会。
The ability to permeate selectively the cell membrane and introduce therapeutic agents is a key goal in cell biology. Optical transfection is a powerful methodology but requires exact focusing due to the required two-photon power density. The authors use a Bessel beam that obviates the need to locate precisely the cell membrane, permitting two-photon excitation along a line leading to cell transfection. Assuming a minimum efficiency of 20%, the Bessel beam offers transfection at axial distances 20 times greater than that of its Gaussian equivalent. Furthermore, the authors demonstrate cell transfection beyond obstacles due to the self-healing nature of the Bessel beam. (C) 2007 American Institute of Physics.