Expression of multidrug resistance type 1 gene (MDR1) P-glycoprotein in intractable epilepsy with different aetiologies: a double-labelling and electron microscopy study
Expression of multidrug resistance type 1 gene (MDR1) P-glycoprotein in intractable epilepsy with different aetiologies: a double-labelling and electron microscopy study
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DOI：
10.1007/s10072-006-0678-8
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发表时间：
2006-09-01
影响因子：
3.3
通讯作者：
Qunyuan, X.
中科院分区：
文献类型：
--
作者：
Chengyun, D.;Guoming, L.;Qunyuan, X.
本研究的目的是分析不同病因的难治性癫痫患者的临床特征、病理特征以及多重耐药1型（MDR1）和胶质纤维酸性蛋白（GFAP）的表达模式，并分析临床与病理结果之间的关系。本研究纳入26例难治性癫痫患者（男15例，女11例，年龄4-25岁，平均年龄22.92岁，SD 11.19岁）；结合临床特点，在光镜和电镜下检查各受试者手术切除脑组织的病理变化以及MDR1和GFAP的表达。所有患者均表现为长期难治性癫痫，大部分为部分型，原因不同，如外伤、血管损伤、脑炎、皮质发育不良、海绵状血管瘤和Sturge-Weber病。神经元退行性损伤、星形胶质细胞的反应性增殖以及 GFAP 和 MDR1 的过度表达是所有病例的共同病理特征。通过电子显微镜检测 MDR1 使我们能够精确确定其在反应性星形胶质细胞中的细胞位置，并排除其他细胞类型中抗原的存在。在所有病例中，光学和电子显微镜下的病理特征都是相似的，与不同的临床表现和病因无关。
The objective of this study was to analyse the clinical characteristics, pathological features and expression patterns of multiple drug resistance type 1 (MDR1) and glial fibrillary acidic protein (GFAP) in intractable epilepsy patients with variable aetiologies and to analyse the relationships between the clinical and pathological findings. Twenty-six patients (15 males, 11 females, age range 4-25 years, mean age 22.92 years, SD 11.19 years) with intractable epilepsy were included in this study; the clinical characteristics were considered, and the pathological changes as well as expression of MDR1 and GFAP in surgically removed brain tissues of each subject were examined under light and electron microscopy. All patients presented a long-lasting, refractory epilepsy, mostly of the partial type, due to different causes, such as trauma, vascular injuries, encephalitis, cortical dysplasia, cavernous angioma and Sturge-Weber disease. Neuronal degenerative damage, reactive proliferation of astrocytes, as well as overexpression of GFAP and MDR1, appeared as common pathological features in all cases. The detection of MDR1 by electron microscopy allowed us to precisely define its cellular location in reactive astrocytes and to exclude the presence of the antigen in other cellular types. In all cases, pathological features, at both light and electron microscopy, were similar, independent of the different clinical presentation and aetiology.