An Injectable Nanocomposite Hydrogel for Potential Application of Vascularization and Tissue Repair
An Injectable Nanocomposite Hydrogel for Potential Application of Vascularization and Tissue Repair
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一种可注射的纳米复合水凝胶，用于血管化和组织修复的潜在应用
DOI：
10.1007/s10439-020-02471-7
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发表时间：
2020-02-07
影响因子：
3.8
通讯作者：
Yang，Ping
中科院分区：
文献类型：
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作者：
Ding，Yilei;Zhao，An-sha;Yang，Ping
在该贡献中，用壳聚糖、明胶、β-甘油磷酸盐和Arg-Gly-Asp（RGD）肽开发了可注射的水凝胶：该水凝胶在室温下为液体，并且在37 ℃下快速凝胶化; RGD肽为各种细胞，特别是内皮细胞（EC）、平滑肌细胞（SMC）和间充质干细胞（MSC）提供了更好的生长微环境。基质细胞衍生因子-1（SDF-1）纳米颗粒和血管内皮生长因子（VEGF）纳米颗粒均装载在可注射水凝胶中，以模拟细胞外基质（ECM）中的天然纳米颗粒，从而促进血管生成。体外EC/SMC和MSC/SMC共培养实验表明，纳米复合水凝胶加速了血管胚胎的构建，鸡胚绒毛尿囊膜模型表明其具有促进细胞迁移和血管再生的能力。将该纳米复合水凝胶注射到大鼠心肌梗死（MI）模型中，结果表明，与对照组相比，大鼠心功能恢复更好。我们希望这种可注射的纳米复合水凝胶在组织工程中有更广泛的应用。
In this contribution, an injectable hydrogel was developed with chitosan, gelatin, β-glycerphosphate and Arg-Gly-Asp (RGD) peptide: this hydrogel is liquid in room temperature and rapidly gels at 37 C; RGD peptide promises better growth microenvironment for various cells, especially endothelial cells (EC), smooth muscle cells (SMC) and mesenchymal stem cells (MSC). Both stromal cell-derived factor-1 (SDF-1) nanoparticle and vascular endothelial growth factor (VEGF) nanoparticles were loaded in the injectable hydrogel to simulate the natural nanoparticles in the extracellular matrix (ECM) to promote angiogenesis. In vitro EC/SMC and MSC/SMC co-culture experiment indicated that the nanocomposite hydrogel accelerated constructing embryonic form of blood vessels, and chick embryo chorioallantoic membrane model demonstrated its ability of improving cells migration and blood vessel regeneration. We injected this nanocomposite hydrogel into rat myocardial infarction (MI) model and the results indicated that the rats heart function recovered better compared control group. We hope this injectable nanocomposite hydrogel may possess wider application in tissue engineering.