Functional genomic analysis identifies miRNA repertoire regulating C. elegans oocyte development.
Functional genomic analysis identifies miRNA repertoire regulating C. elegans oocyte development.
复制标题
功能基因组分析鉴定了调节线虫卵母细胞发育的 miRNA 库。
DOI：
10.1038/s41467-018-07791-w
复制
发表时间：
2018
影响因子：
16.6
通讯作者：
Arur,Swathi
中科院分区：
文献类型：
--
作者：
Minogue,AmandaL;Tackett,MichaelR;Atabakhsh,Elnaz;Tejada,Genesis;Arur,Swathi
Oocyte-specific miRNA function remains unclear in mice and worms because loss of Dgcr8 and Dicer from mouse and worm oocytes, respectively, does not yield oogenic defects. These data lead to several models: (a) miRNAs are not generated in oocytes; (b) miRNAs are generated but do not perform an oogenic function; (c) functional oocyte miRNAs are generated in a manner independent of these enzymes. Here, we test these models using a combination of genomic, expression and functional analyses on theC.elegansgermline. We identify a repertoire of at least twenty-three miRNAs that accumulate in four spatial domains in oocytes. Genetic tests demonstrate that oocyte-expressed miRNAs regulate key oogenic processes within their respective expression domains. Unexpectedly, we find that over half of the oocyte-expressed miRNAs are generated through an unknown Drosha independent mechanism. Thus, a functional miRNA repertoire generated via Drosha dependent and independent pathways regulatesC.elegansoocyte development.
