Calcium spikes, waves and oscillations in a large, patterned epithelial tissue
Calcium spikes, waves and oscillations in a large, patterned epithelial tissue
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DOI：
10.1038/srep42786
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发表时间：
2017-02-20
影响因子：
4.6
通讯作者：
Classen, Anne-Kathrin
中科院分区：
文献类型：
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作者：
Balaji, Ramya;Bielmeier, Christina;Classen, Anne-Kathrin
虽然钙信号在兴奋性细胞，如肌肉或神经元，被广泛的特点，钙信号在上皮组织中的了解甚少。具体而言，由紧密耦合的上皮细胞引起的细胞间钙信号传导模式的范围及其在上皮特性调节中的功能很少探索。我们发现，在果蝇成虫盘，一个广泛研究的上皮模型器官，复杂的时空钙动力学发生。我们描述的模式，包括细胞间波穿越大的组织领域在引人注目的振荡模式，以及尖峰局限于本地域的相邻细胞。细胞间波和振荡的时空特性出现作为紧急性能的钙动员内的一张缝隙连接耦合的细胞，并受到细胞大小和环境的历史。虽然在体内功能的尖峰，波和振荡需要进一步表征，我们的遗传实验表明，核心钙信号传导组件指导肌动球蛋白组织。因此，我们的研究表明钙信号在上皮细胞中的可能作用，但重要的是，引入了一种模型上皮，能够解剖支持长距离细胞增殖的启动、传递和再生的细胞机制。
While calcium signaling in excitable cells, such as muscle or neurons, is extensively characterized, calcium signaling in epithelial tissues is little understood. Specifically, the range of intercellular calcium signaling patterns elicited by tightly coupled epithelial cells and their function in the regulation of epithelial characteristics are little explored. We found that in Drosophila imaginal discs, a widely studied epithelial model organ, complex spatiotemporal calcium dynamics occur. We describe patterns that include intercellular waves traversing large tissue domains in striking oscillatory patterns as well as spikes confined to local domains of neighboring cells. The spatiotemporal characteristics of intercellular waves and oscillations arise as emergent properties of calcium mobilization within a sheet of gap-junction coupled cells and are influenced by cell size and environmental history. While the in vivo function of spikes, waves and oscillations requires further characterization, our genetic experiments suggest that core calcium signaling components guide actomyosin organization. Our study thus suggests a possible role for calcium signaling in epithelia but importantly, introduces a model epithelium enabling the dissection of cellular mechanisms supporting the initiation, transmission and regeneration of long-range