The effects of gibberellin on the expression of symbiosis-related genes in Paris-type arbuscular mycorrhizal symbiosis in Eustoma grandiflorum
The effects of gibberellin on the expression of symbiosis-related genes in Paris-type arbuscular mycorrhizal symbiosis in Eustoma grandiflorum
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DOI：
10.1080/15592324.2020.1784544
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发表时间：
2020-06
影响因子：
2.9
通讯作者：
Takaya Tominaga;K. Yamaguchi;S. Shigenobu;M. Yamato;H. Kaminaka
中科院分区：
文献类型：
--
作者：
Takaya Tominaga;K. Yamaguchi;S. Shigenobu;M. Yamato;H. Kaminaka
摘要丛枝菌根(AM)是一种在陆地植物中的共生相互作用，由真菌在球菌纲中定植。AM的形态类型，包括Arum类型和Paris类型，根据寄主植物的不同而不同。Arum型AM共生的部分调控途径已被揭示，因为大多数模式植物与一种模式AM真菌--不规则根腐真菌形成Arum型AM。此外，赤霉素(GA)在Arum型AM共生中能严重抑制AM真菌的定植。最近，我们发现外源赤霉素GA处理显著促进了AM真菌在巴黎型AM共生中的定植。在本研究中，我们重点研究了赤霉素处理的桔梗AM共生相关基因的转录变化。与对照相比，GA处理维持或提高了所有被检测基因的表达水平。我们的新结果表明，在桔梗中建立AM共生所需的信号通路(S)可能不同于模式植物中已有的信号通路。
ABSTRACT Arbuscular mycorrhiza (AM) is a symbiotic interaction in terrestrial plants that is colonized by fungi in the Glomeromycotina. The morphological types of AM, including the Arum-type and Paris-type, are distinct, depending on the host plant species. A part of the regulatory pathways in Arum-type AM symbiosis has been revealed because most model plants form the Arum-type AM with a model AM fungus, Rhizophagus irregularis. Moreover, gibberellin (GA) is known to severely inhibit AM fungal colonization in Arum-type AM symbiosis. Recently, we showed that exogenous GA treatment significantly promoted AM fungal colonization in Paris-type AM symbiosis in Eustoma grandiflorum. In this study, we focused on the transcriptional changes in AM symbiosis-related genes in GA-treated E. grandiflorum. The expression levels of all examined E. grandiflorum genes were maintained or increased by GA treatment compared with those of the control treatment. Our new results suggest that signaling pathway(s) required for establishing AM symbiosis in E. grandiflorum may be distinct from the well-characterized pathway for that in model plants.