SUPPORT OF CULTURED-HEPATOCYTES BY A LAMININ-RICH GEL - EVIDENCE FOR A FUNCTIONALLY SIGNIFICANT SUBENDOTHELIAL MATRIX IN NORMAL RAT-LIVER
SUPPORT OF CULTURED-HEPATOCYTES BY A LAMININ-RICH GEL - EVIDENCE FOR A FUNCTIONALLY SIGNIFICANT SUBENDOTHELIAL MATRIX IN NORMAL RAT-LIVER
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DOI：
10.1172/jci112887
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发表时间：
1987-03-01
影响因子：
15.9
通讯作者：
ROLL, FJ
中科院分区：
文献类型：
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作者：
BISSELL, DM;ARENSON, DM;ROLL, FJ
免疫组织化学研究表明，正常大鼠肝内皮下间隙含有基底膜的组成蛋白。然而，目前尚不清楚这些蛋白质是否构成了影响肝细胞功能的复合体。我们用培养在基质蛋白底物上的正常大鼠肝细胞作为细胞和基底膜相互作用的活体模型来研究这个问题。在I型胶原基质上培养的细胞，白蛋白分泌在2d后逐渐减少。相反，当细胞培养在富含层粘连蛋白的凝胶基质上时，白蛋白分泌至少稳定3wk，其他功能和超微结构也同样保持不变。没有一种单独的基质蛋白有效地替代凝胶基质，这表明完全支持肝细胞功能需要基质蛋白的复合体。我们推测，急性炎症时肝细胞功能障碍的原因之一是基质的破坏及其与肝细胞质膜相互作用的改变。
The subendothelial space of normal rat liver contains the constituent proteins of a basal lamina, as judged by immunohistochemical study of tissue sections. However, it is unknown whether these proteins constitute a complex with effects on hepatocellular function. We have examined this question, using normal rat hepatocytes cultured on substrata of matrix proteins as a model of the interaction between cells and basal lamina in vivo. In cultures on a type I collagen substratum, albumin secretion decreased progressively after 2 d. By contrast, when cells were cultured on a laminin-rich gel matrix, albumin secretion was stable for a least 3 wk; other functions and ultrastructural morphology were similarly maintained. None of the individual matrix proteins effectively substituted for the gel matrix, suggesting that full support of hepatocellular function requires a complex of matrix proteins. We speculate that a cause of hepatocellular dysfunction in acute inflammation is disruption of this matrix and alteration of its interaction with the hepatocyte plasma membrance.