POLLEN CHROMOSOME DOUBLING UNDER HIGH TEMPERATURE IN Populus alba L.
POLLEN CHROMOSOME DOUBLING UNDER HIGH TEMPERATURE IN Populus alba L.
复制标题
DOI：
--
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Kang Xiangyang
中科院分区：
文献类型：
--
作者：
Kang Xiangyang
在观察银白杨花粉减数分裂过程的基础上，对其高温诱导花粉染色体加倍技术进行了研究。结果表明，银白杨花粉母细胞减数分裂过程持续3~4d，减数分裂阶段与花药颜色有关，高温诱导花粉染色体加倍的最佳时期为中期I期，36℃处理4h，2n花粉出现率达39.6%；高温处理延缓了花药裂开，花药裂开比未处理花药晚2~3d，花药中几乎没有收集到花粉。
Techniques of high temperature-induced pollen chromosome doubling in Populus alba L.were studied based on observation of its meiotic process.The results showed that the meiotic process of pollen mother cells lasted for 3～4 d,and there was a relationship between the meiotic stages and color of anthers.The best stage for high temperature-induced pollen chromosome doubling was metaphase I.Frequency of 2n pollen was up to 39.6% at treatment of 36℃ for 4h,and the mean frequency of induced 2n pollen was 32.75%.Anther dehiscence was delayed owing to the effect of high temperature treatment.The dehiscence of treated anthers was 2～3d later than that of the untreated anthers,and just little pollen was collected from the treated anthers.