Generation of NANOS3-mCherry reporter human embryonic stem cell line SYSUe-009-A using CRISPR/Cas9
Generation of NANOS3-mCherry reporter human embryonic stem cell line SYSUe-009-A using CRISPR/Cas9
复制标题
使用 CRISPR/Cas9 生成 NANOS3-mCherry 报告基因人胚胎干细胞系 SYSUe-009-A
DOI：
10.1016/j.scr.2023.103022
复制
发表时间：
2023
影响因子：
1.2
通讯作者：
Chuanbo Sun
中科院分区：
文献类型：
--
作者：
Ruirui Guo;Simin Yuan;Bing Li;Jichang Wang;Chuanbo Sun
NANOS 3是一种含有锌指的RNA结合蛋白，已被证明在人类原始生殖细胞（hPGC）中高度表达，因此NANOS 3可以作为hPGC发育的标志物。由于伦理和技术的限制，获得原代人类生殖系细胞非常困难。由多能干细胞诱导产生的人原始生殖细胞样细胞（human primordial germ cell-like cells，hPGCLC）是研究人类生殖细胞的理想选择。这种在NANOS 3终止密码子之前的位点敲入mCherry的hESC系可以作为学习人类PGC特异性的有用工具。
NANOS3 is a zinc-finger containing RNA-binding protein that has been demonstrated to be highly expressed in human primordial germ cell(hPGC), thus NANOS3 can serve as a marker for hPGC development. Due to the ethical and technical restrictions, it is difficult to get primary human germline cells. Human primordial germ cell-like cells (hPGCLCs) generated from pluripotent stem cells is an excellent alternatives in human germ cell-related studies. This hESC line with an mCherry knock-in at the site before NANOS3′s stop codon serves as a useful tool to learn human PGC specification.