Direct methylation from mouse plasma and from liver and brain homogenates
Direct methylation from mouse plasma and from liver and brain homogenates
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DOI：
10.1016/j.ab.2005.04.037
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发表时间：
2005-08-01
影响因子：
2.9
通讯作者：
Wada, S
中科院分区：
文献类型：
--
作者：
Shirai, N;Suzuki, H;Wada, S
血浆和组织的脂肪酸组成分析作为研究脂质营养的重要方法。我们研究了直接甲基化的脂肪酸的可能性BF 3-甲醇从血浆和肝脏和脑匀浆，而无需脂质提取。直接甲基化方法产生的色谱图中没有鬼峰。18：0百分比在直接甲基化方法中显著高于脂质提取方法。直接甲基化法和冷冻干燥后甲基化法的脂肪酸组成无显著差异。直接甲基化法的内标回收率高于脂质萃取法。脂肪酸组成与油脂提取物的差异可能是由于油脂类提取物的变化造成的。因此，不提取脂质的直接甲基化法最适合测定血浆和组织中的脂肪酸组成。(c)2005年爱思唯尔公司All rights reserved.
The analysis of fatty acid composition of plasma and tissue is important as a method for studying lipid nutrition. We investigated the possibility of direct methylation of fatty acids by BF3-methanol from plasma and from liver and brain homogenates without lipid extraction. There were no ghost peaks in the chromatogram produced by the direct methylation method. The 18:0 percentages were significantly higher in the direct methylation method than in the lipid extraction method. There were not remarkable differences in fatty acid composition in the direct methylation and methylation after lyophilization methods. Furthermore, the recovery ratio of the internal standard in the direct methylation, method was higher than that in the lipid extraction method. The difference of fatty acid composition with lipid extraction may be caused by the change of lipid class extraction. Therefore, the direct methylation method without lipid extraction is the most suitable for determining fatty acid composition in plasma and tissue. (c) 2005 Elsevier Inc. All rights reserved.