Fast subcellular optical coherence photoacoustic microscopy for pigment cell imaging.
Fast subcellular optical coherence photoacoustic microscopy for pigment cell imaging.
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DOI：
10.1364/ol.40.004448
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发表时间：
2015-10
期刊：
影响因子：
3.6
通讯作者：
Lin Li;Cuixia Dai;Qian Li;Qingliang Zhao;Xiaoyun Jiang;X. Chai;Chuanqing Zhou
中科院分区：
文献类型：
--
作者：
Lin Li;Cuixia Dai;Qian Li;Qingliang Zhao;Xiaoyun Jiang;X. Chai;Chuanqing Zhou
我们将光学相干显微镜（OCM）和光声显微镜（PAM）两种互补成像模式相结合，开发了一种用于细胞/亚细胞成像的快速超高分辨率光学相干光声显微镜（FU-OCPAM）系统。该系统采用光学扫描技术实现快速成像，OCM和PAM的分辨率分别为1.24 μm和0.59 μm。我们对视网膜色素上皮（RPE）进行成像，以证明FU-OCPAM系统的亚细胞成像能力。OCM和PAM图像清晰地显示了RPE细胞的形态，反映了散射和吸收互补的光学特性。利用光声（PA）信号对RPE细胞进行定量分析。细胞面积主要在80 ~ 300 μm2之间，与主要反映细胞黑色素含量的PA信号强度和呈线性关系。形态学和PA信号可以定性和定量地识别RPE细胞的衰老和健康状态。结果表明，FU-OCPAM在细胞水平上研究细胞对外部刺激的实时反应以及衰老和疾病的进展方面具有潜在的应用价值。
We developed a fast ultrahigh resolution optical coherence photoacoustic microscopy (FU-OCPAM) system by combining two complementary imaging modes of optical coherence microscopy (OCM) and photoacoustic microscopy (PAM) for cellular/subcellular imaging. The system used optical scanning to realize fast imaging speed and provided ultrahigh resolution of 1.24 and 0.59 μm for OCM and PAM, respectively. We imaged the retinal pigment epithelium (RPE) to demonstrate the subcellular imaging capability of the FU-OCPAM system. The OCM and PAM images clearly showed the RPE cell morphology and reflected the complementary optical properties of scattering and absorption. A quantitative analysis of the RPE cells was made based on photoacoustic (PA) signals. The cell area mainly ranged from 80 to 300 μm2, and had a linear relationship with the sum intensity of PA signals which mainly reflected the melanin content of the cells. The morphology and the PA signal could be used to identify qualitatively and quantitatively the aging and healthy states of the RPE cells. The results show the potential applications in studying the real-time cellular response to external stimulations and the progress of aging and diseases at the cellular level with FU-OCPAM.