Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering
Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering
复制标题
DOI：
10.3390/mi11070679
复制
发表时间：
2020-07
期刊：
影响因子：
3.4
通讯作者：
Uran Watanabe;S. Sugiura;M. Kakehata;F. Yanagawa;T. Takagi;K. Sumaru;T. Satoh;M. Tamura;Y. Hosokawa;K. Torizuka;T. Kanamori
中科院分区：
文献类型：
--
作者：
Uran Watanabe;S. Sugiura;M. Kakehata;F. Yanagawa;T. Takagi;K. Sumaru;T. Satoh;M. Tamura;Y. Hosokawa;K. Torizuka;T. Kanamori
工程血管在体外可以概括血管的功能，可以作为一种新的微生理系统应用于药物研发。近年来，在水凝胶中构建血管模型的各种方法被报道用于体外血管功能的研究，然而，通常情况下，很难制造出具有设计的尺寸和结构的中空结构来用于血管组织工程。本研究报道了一种在微流控装置中制备光降解水凝胶中空结构的方法。红外飞秒脉冲激光通过多光子激发诱导光降解，在水凝胶中以程序控制的方式扫描，以制备所设计的中空结构。通过叠氮改性明胶溶液与端二苯并环辛基可光裂解四臂聚乙二醇交联剂溶液的交联反应，制备了光降解水凝胶。在评价了光降解水凝胶的溶胀性和细胞粘附性后，对微流控装置中制备的水凝胶进行了激光扫描处理，得到了线状和支化的中空结构。我们将微球悬浮液引入到光降解水凝胶的制备结构中，并通过多光子激发过程证实了所设计图案的可灌装中空结构的制备。
Engineered blood vessels generally recapitulate vascular function in vitro and can be utilized in drug discovery as a novel microphysiological system. Recently, various methods to fabricate vascular models in hydrogels have been reported to study the blood vessel functions in vitro; however, in general, it is difficult to fabricate hollow structures with a designed size and structure with a tens of micrometers scale for blood vessel tissue engineering. This study reports a method to fabricate the hollow structures in photodegradable hydrogels prepared in a microfluidic device. An infrared femtosecond pulsed laser, employed to induce photodegradation via multi-photon excitation, was scanned in the hydrogel in a program-controlled manner for fabricating the designed hollow structures. The photodegradable hydrogel was prepared by a crosslinking reaction between an azide-modified gelatin solution and a dibenzocyclooctyl-terminated photocleavable tetra-arm polyethylene glycol crosslinker solution. After assessing the composition of the photodegradable hydrogel in terms of swelling and cell adhesion, the hydrogel prepared in the microfluidic device was processed by laser scanning to fabricate linear and branched hollow structures present in it. We introduced a microsphere suspension into the fabricated structure in photodegradable hydrogels, and confirmed the fabrication of perfusable hollow structures of designed patterns via the multi-photon excitation process.