Cloning and expression of the ferredoxin gene from extremely halophilic archaeon Haloarcula japonica strain TR-1
Cloning and expression of the ferredoxin gene from extremely halophilic archaeon Haloarcula japonica strain TR-1
复制标题
极嗜盐古菌Haloarcula japonica TR-1株铁氧还蛋白基因的克隆与表达
DOI：
10.1023/a:1016625916878
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发表时间：
2001
期刊：
影响因子：
3.5
通讯作者：
Satoshi Nakamura
中科院分区：
文献类型：
--
作者：
T. Matsuo;A. Ikeda;Hiroto Seki;T. Ichimata;D. Sugimori;Satoshi Nakamura
克隆并测定了大梭鱼(Haloarcula Japan)tr-1铁氧还蛋白(Fd)的基因序列。克隆的Ha基因的序列分析。该结构基因由387个核苷酸组成，编码129个氨基酸。推导出Ha的氨基酸序列。JAPONICAFd与其他极端嗜盐古菌的相应序列有84%到98%的同源性。TheHa.将日本血吸虫Fd基因插入穿梭载体pWL102，转化Ha。日本。哈。然后，可以将日本血吸虫Fd与他的⋅标签(6xHis)Inha融合生产。日本宿主细胞。Fd/His⋅标签融合蛋白的吸收光谱和电子自旋共振光谱表明，存在一个具有天然Ha特征的[2Fe-2S]簇。日本血吸虫。
The gene encoding a ferredoxin (Fd) fromHaloarcula japonicastrain TR-1 was cloned and sequenced. Sequence analysis of the clonedHa. japonicaFd gene revealed that the structural gene consisted of an open reading frame of 387 nucleotides encoding 129 amino acids. The deduced amino acid sequence ofHa. japonicaFd showed 84 to 98% identity with corresponding sequences in other extremely halophilic archaea. TheHa. japonicaFd gene was inserted into the shuttle vector pWL102 and used to transformHa. japonica.Ha. japonicaFd could then be produced as a fusion with His⋅Tag (6xHis) inHa. japonicahost cells. The absorption and ESR spectra of the Fd/His⋅Tag fusion protein revealed the presence of a [2Fe-2S] cluster which is characteristic of nativeHa. japonicaFd.