Functional characterization of GmBZL2 (AtBZR1 like gene) reveals the conserved BR signaling regulation in Glycine max.
Functional characterization of GmBZL2 (AtBZR1 like gene) reveals the conserved BR signaling regulation in Glycine max.
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GmBZL2（AtBZR1 样基因）的功能表征揭示了大豆中保守的 BR 信号调节
DOI：
10.1038/srep31134
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发表时间：
2016-08-08
影响因子：
4.6
通讯作者：
Lin WH
中科院分区：
文献类型：
--
作者：
Zhang Y;Zhang YJ;Yang BJ;Yu XX;Wang D;Zu SH;Xue HW;Lin WH
类甾醇（BRs）在植物生长发育中起着重要作用，并调节着多种农艺性状。增强的BR信号导致拟南芥bzr 1 -1D（AtBZR 1 P234 L，BR信号/效应中重要转录因子的功能获得性突变体）的种子数量和产量增加。BR信号转导途径在拟南芥中已被阐明，但在其他物种中知之甚少。大豆是一种重要的双子叶植物，生产食用油和蛋白质。系统进化分析表明AtBZR 1类基因在被子植物中高度保守，在大豆中有4个同源基因（GmBZL 1 -4）。我们在这里报告GmBZL 2（在花中相对高度表达）的功能特性。AtBZR 1的P234位点在GmBZL 2（P216）中是保守的，GmBZL 2 P216 L突变导致GmBZL 2积累。GmBZL 2 P216 L（GmBZL 2 *）在拟南芥中导致增强的BR信号传导;包括增加的每长角果种子数。GmBZL 2 * 部分修复了bri 1 -5的缺陷，进一步证明了GmBZL 2与AtBZR 1的保守功能。BR处理促进了GmBZL 2的积累、核定位和去磷酸化/磷酸化比率，表明GmBZL 2活性受到BR信号的保守调节。本研究不仅揭示了GmBZL 2和BR信号通路在大豆中的保守调控机制，而且揭示了GmBZL 2在大豆种子产量方面的潜在应用。
Brassinosteroids (BRs) play key roles in plant growth and development, and regulate various agricultural traits. Enhanced BR signaling leads to increased seed number and yield in Arabidopsis bzr1-1D (AtBZR1P234L, gain-of-function mutant of the important transcription factor in BR signaling/effects). BR signal transduction pathway is well elucidated in Arabidopsis but less known in other species. Soybean is an important dicot crop producing edible oil and protein. Phylogenetic analysis reveals AtBZR1-like genes are highly conserved in angiosperm and there are 4 orthologues in soybean (GmBZL1-4). We here report the functional characterization of GmBZL2 (relatively highly expresses in flowers). The P234 site in AtBZR1 is conserved in GmBZL2 (P216) and mutation of GmBZL2P216L leads to GmBZL2 accumulation. GmBZL2P216L (GmBZL2*) in Arabidopsis results in enhanced BR signaling; including increased seed number per silique. GmBZL2* partially rescued the defects of bri1-5, further demonstrating the conserved function of GmBZL2 with AtBZR1. BR treatment promotes the accumulation, nuclear localization and dephosphorylation/phosphorylation ratio of GmBZL2, revealing that GmBZL2 activity is regulated conservatively by BR signaling. Our studies not only indicate the conserved regulatory mechanism of GmBZL2 and BR signaling pathway in soybean, but also suggest the potential application of GmBZL2 in soybean seed yield.