ORA47 (octadecanoid-responsive AP2/ERF-domain transcription factor 47) regulates jasmonic acid and abscisic acid biosynthesis and signaling through binding to a novel cis-element
ORA47 (octadecanoid-responsive AP2/ERF-domain transcription factor 47) regulates jasmonic acid and abscisic acid biosynthesis and signaling through binding to a novel cis-element
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DOI：
10.1111/nph.13914
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发表时间：
2016-07-01
期刊：
影响因子：
9.4
通讯作者：
Lin, Tsan-Piao
中科院分区：
文献类型：
--
作者：
Chen, Hsing-Yu;Hsieh, En-Jung;Lin, Tsan-Piao
ARA 47（十八烷酸响应性AP 2/ERF结构域转录因子47）是拟南芥的AP 2/ERF结构域转录因子，调节茉莉酸（JA）生物合成，并由甲基JA处理诱导。ORA 47的调控机制尚不清楚。ORA 47被证明与ABI 2启动子中的顺式元件（NC/GT）CGNCCA结合，该元件被称为O盒。我们推测，ORA 47是阿坝insensitive 1（ABI 1）和ABI 2之间的连接蛋白，并介导ABI 1-ORA 47-ABI 2正反馈环。具体而言，许多脱落酸（阿坝）和JA生物合成和信号传导基因是ORA 47在胁迫条件下的直接靶点。相对于野生型植物，P35 S：ORA 47-GR系的JA含量在创伤下被高度诱导，在水分胁迫下被中度诱导。结果表明，外源基因ORA 47在植物遭受伤害和水分胁迫时，可能参与JA和阿坝的生物合成，并调控一系列激素基因的合成和/或信号转导。
ORA47 (octadecanoid-responsive AP2/ERF-domain transcription factor 47) of Arabidopsis thaliana is an AP2/ERF domain transcription factor that regulates jasmonate (JA) biosynthesis and is induced by methyl JA treatment. The regulatory mechanism of ORA47 remains unclear. ORA47 is shown to bind to the cis-element (NC/GT) CGNCCA, which is referred to as the O-box, in the promoter of ABI2. We proposed that ORA47 acts as a connection between ABA INSENSITIVE1 (ABI1) and ABI2 and mediates an ABI1-ORA47-ABI2 positive feedback loop.PORA47: ORA47-GFP transgenic plants were used in a chromatin immunoprecipitation (ChIP) assay to show that ORA47 participates in the biosynthesis and/or signaling pathways of nine phytohormones. Specifically, many abscisic acid (ABA) and JA biosynthesis and signaling genes were direct targets of ORA47 under stress conditions.The JA content of the P35S: ORA47-GR lines was highly induced under wounding and moderately induced under water stress relative to that of the wild-type plants. The wounding treatment moderately increased ABA accumulation in the transgenic lines, whereas the water stress treatment repressed the ABA content.ORA47 is proposed to play a role in the biosynthesis of JA and ABA and in regulating the biosynthesis and/or signaling of a suite of phytohormone genes when plants are subjected to wounding and water stress.