Development of a Selective Medium for Isolation ofHelicobacter pylori from Cattle and Beef Samples
Development of a Selective Medium for Isolation ofHelicobacter pylori from Cattle and Beef Samples
复制标题
开发用于从牛和牛肉样品中分离幽门螺杆菌的选择性培养基
DOI：
10.1128/aem.66.2.723-727.2000
复制
发表时间：
2000
影响因子：
4.4
通讯作者：
G. Acuff
中科院分区：
文献类型：
--
作者：
T. Stevenson;L. M. Lucia;G. Acuff
摘要 幽门螺杆菌已从人胃中分离出来，培养基仅含有极少量的选择剂。然而，由于环境、口腔和粪便样本中污染物数量较多，目前对感染传播和来源的研究需要更具选择性的介质。本研究的目的是开发和评估具有足够选择性的检测技术，以从潜在的动物和食物来源中分离出幽门螺杆菌。由于幽门螺杆菌在胃的酸性环境中生存，因此研究了添加尿素的低 pH 值作为潜在的选择性组合。幽门螺杆菌在补充有 10 mM 尿素、pH 4.5 的幽门螺杆菌特殊蛋白胨平板培养基上生长得相当好，但该 pH 值并不能充分抑制污染物的生长。然后比较了各种抗生素组合，由每升 10 mg 万古霉素、每升 5 mg 两性霉素 B、每升 10 mg 头孢磺啶、每升 62,000 IU 硫酸多粘菌素 B、每升 40 mg 甲氧苄啶和每升磺胺甲恶唑 20 mg 组成的组合被证明具有高度选择性，但仍能产生强大的幽门螺杆菌菌落成长。该培养基对于从牛和牛肉样品中回收幽门螺杆菌具有高度选择性，并且有可能用于增强从可能被大量竞争性微生物污染的人类和环境样品中回收该细菌。
ABSTRACT Helicobacter pylori has been isolated from the human stomach with media containing only minimal selective agents. However, current research on the transmission and sources of infection requires more selective media due to the higher numbers of contaminants in environmental, oral, and fecal samples. The objective of this study was to develop and evaluate detection techniques that are sufficiently selective to isolate H. pylori from potential animal and food sources. Since H. pylori survives in the acidic environment of the stomach, low pH with added urea was studied as a potential selective combination. H. pylori grew fairly well on H. pylori Special Peptone plating medium supplemented with 10 mM urea at pH 4.5, but this pH did not sufficiently inhibit the growth of contaminants. Various antibiotic combinations were then compared, and a combination consisting of 10 mg of vancomycin per liter, 5 mg of amphotericin B per liter, 10 mg of cefsulodin per liter, 62,000 IU of polymyxin B sulfate per liter, 40 mg of trimethoprim per liter, and 20 mg of sulfamethoxazole per liter proved to be highly selective but still allowed robust colonies of H. pylori to grow. This medium was highly selective for recovering H. pylori from cattle and beef samples, and it is possible that it could be used to enhance the recovery of this bacterium from human and environmental samples, which may be contaminated with large numbers of competing microorganisms.