Evaluating the neural basis of temporal order memoryfor visual stimuli in the rat
Evaluating the neural basis of temporal order memoryfor visual stimuli in the rat
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DOI：
10.1111/j.1460-9568.2010.07555.x
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发表时间：
2011-02-01
影响因子：
3.4
通讯作者：
Warburton, E. C.
中科院分区：
文献类型：
--
作者：
Barker, G. R. I.;Warburton, E. C.
时间顺序记忆（刺激顺序记忆）对于熟悉物体之间的区分至关重要，并且依赖于涉及嗅周皮层（PRH）和内侧前额叶皮层的神经回路。本研究探讨了海马能和胆碱能神经传递在编码或检索的时间顺序记忆的作用，使用的任务，要求动物区分两个熟悉的对象，在不同的时间间隔。在采样阶段2之前（在编码期间具有活性）或测试之前（在检索期间具有活性），给予6-氰基-7-硝基喹喔啉（CNQX）（AMPA/红藻氨酸受体拮抗剂）、东莨菪碱（毒蕈碱受体拮抗剂）或2-氨基-5-膦酰基戊酸（AP 5）（N-甲基-D-天冬氨酸受体拮抗剂）。单侧CNQX给药至相对半球的PRH和前边缘/下边缘皮质（PL/IL），即破坏回路内的神经传递，损害编码和提取。东莨菪碱或AP 5在PRH-PL/IL回路中的给药损害了编码。然后分别研究每个脑区的药物作用。内PRH CNQX，东莨菪碱或AP 5中断编码，使动物探索最近的对象显着超过旧的对象。相比之下，内PL/IL CNQX，东莨菪碱或AP 5损害记忆性能，使得动物花费相同的时间探索物体。CNQX而非AP 5或东莨菪碱损害提取。此外，CNQX损害新的对象偏好时，注入PRH，而不是PL/IL后3小时的延迟。因此，编码的时间顺序记忆是介导的塑料过程涉及N-甲基-D-天冬氨酸和毒蕈碱受体的PRH-PL/IL电路内，但这两个地区作出了质的不同的认知贡献，形成这个记忆过程。
Temporal order memory (memory for stimulus order) is crucial for discrimination between familiar objects and depends upon a neural circuit involving the perirhinal cortex (PRH) and medial pre-frontal cortex. This study examined the role of glutamatergic and cholinergic neurotransmission in the encoding or retrieval of temporal order memory, using a task requiring the animals to discriminate between two familiar objects presented at different intervals. 6-Cyano-7-nitroquinoxaline (CNQX) (AMPA/kainate receptor antagonist), scopolamine (muscarinic receptor antagonist) or 2-amino-5-phosphonopentanoic acid (AP5) (N-methyl-D-aspartate receptor antagonist) was administered before sample phase 2 (to be active during encoding) or before test (to be active during retrieval). Unilateral CNQX administration into the PRH and pre-limbic/infra-limbic cortices (PL/IL) in opposite hemispheres, i.e. to disrupt neurotransmission within the circuit, impaired encoding and retrieval. Administration of scopolamine or AP5 in the PRH-PL/IL circuit impaired encoding. Drug effects in each brain region were then investigated separately. Intra-PRH CNQX, scopolamine or AP5 disrupted encoding, such that the animals explored the recent object significantly more than the old object. In contrast, intra-PL/IL CNQX, scopolamine or AP5 impaired memory performance such that the animals spent an equal amount of time exploring the objects. CNQX but not AP5 or scopolamine impaired retrieval. Furthermore, CNQX impaired novel object preference when infused into the PRH but not PL/IL following a 3 h delay. Thus, encoding of temporal order memory is mediated by plastic processes involving N-methyl-D-aspartate and muscarinic receptors within the PRH-PL/IL circuit, but these two regions make qualitatively different cognitive contributions to the formation of this memory process.