NEUROPEPTIDE Y BIOSYNTHESIS IS MARKEDLY INDUCED IN MOSSY FIBERS DURING TEMPORAL-LOBE EPILEPSY OF THE RAT
NEUROPEPTIDE Y BIOSYNTHESIS IS MARKEDLY INDUCED IN MOSSY FIBERS DURING TEMPORAL-LOBE EPILEPSY OF THE RAT
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DOI：
10.1016/0304-3940(90)90193-d
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发表时间：
1990-05-04
影响因子：
2.5
通讯作者：
SPERK, G
中科院分区：
文献类型：
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作者：
MARKSTEINER, J;ORTLER, M;SPERK, G
本文观察了海人酸致痫和戊四氮点燃后大鼠海马神经肽Y(NPY)免疫反应和基因表达的变化。在两种动物模型中，苔藓纤维终末区域的NPY免疫反应均明显增强。在海人酸处理的大鼠，在注射毒素后5-60天，这种多肽逐渐积聚在CA3的门区和透明层，在以后的时间间隔，延伸到齿状回的颗粒上分子层，表明这些神经元正在萌芽。单侧肝门注射秋水仙碱后，苔藓纤维的NPY染色消失。原位杂交发现，在两种动物模型中，在含有苔藓纤维包被的颗粒层中，前NPY基因的表达均显著增强。提示癫痫时，神经调节神经肽NPY的持续表达，加上所观察到的可塑性改变，可能是导致海马苔藓纤维兴奋性改变的原因之一。颗粒细胞/苔藓纤维生长抑素免疫反应及基因表达均未见增强。
Neuropeptide Y (NPY) immunoreactivity and gene expression was investigated in the hippocampus after kainic acid-induced seizures and pentylenetetrazol kindling in the rat. Pronounced increases of NPY immunoreactivity were found in the terminal field of mossy fibers in both animal models. In kainic acidtreated rats the peptide progressively accumulated in the hilus and the stratum lucidum of CA3, 5–60 days after injection of the toxin and, at the later intervals, extended to the supragranular molecular layer of the dentate gyrus indicating sprouting of these neurons. Unilateral injection of colchicine into the hilus abolished NPY staining of the mossy fibers. Using in situ hybridization, in both animal models markedly enhanced expression of prepro-NPY mRNA was observed in the granular layer, containing the perikarya of the mossy fibers. It is suggested that sustained expression of the neuromodulatory neuropeptide NPY, in addition to the observed plastic changes, may contribute to altered excitability of hippocampal mossy fibers in epilepsy. Neither somatostatin immunoreactivity nor gene expression were enhanced in granule cells/mossy fibers.