Identification and functional analysis of ferritin 2 from the Taiga tick Ixodes persulcatus Schulze
Identification and functional analysis of ferritin 2 from the Taiga tick Ixodes persulcatus Schulze
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DOI：
10.1016/j.ttbdis.2020.101547
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发表时间：
2020-11-01
影响因子：
3.2
通讯作者：
Ohashi, Kazuhiko
中科院分区：
文献类型：
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作者：
Githaka, Naftaly Wang'ombe;Konnai, Satoru;Ohashi, Kazuhiko
铁蛋白2（FER 2）是一种铁储存蛋白，其已被证明对蜱的吸血和繁殖期间的铁稳态至关重要，因此适合作为抗蜱疫苗的组分。在这项研究中，我们确定了全沟硬蜱的FER 2，这是日本莱姆病和蜱传回归热等人畜共患疾病的主要载体，并研究了其功能。全沟硬蜱铁蛋白2（IpFER 2）具有浓度依赖性的铁结合能力和高度的氨基酸保守性，与其他蜱种的FER 2一致。含有重组Ip-FER 2的疫苗引起成年I.全沟的有趣的是，在同域分布的卵圆硬蜱（Ixodes ovatus）中也观察到饱胀体重的减少。全沟的对I. persulcatus等蜱类与I. sccupularis和I.与长角血蜱（Haemaphysalis longicorras）、微小扇头蜱（Rhipicephalus microplus）和扇头蜱（R.无柄的此外，观察到蜱FER 2序列在一级结构内具有保守区域，并且计算机表位作图分析显示抗原区域也是保守的，特别是在硬蜱属蜱中。总之，数据支持使用本文鉴定的Ip-FER 2抗原的进一步保护性蜱接种应用。
Ferritin 2 (FER2) is an iron storage protein, which has been shown to be critical for iron homeostasis during blood feeding and reproduction in ticks and is therefore suitable as a component for anti-tick vaccines. In this study, we identified the FER2 of Ixodes persulcatus, a major vector for zoonotic diseases such as Lyme borreliosis and tick-borne relapsing fever in Japan, and investigated its functions. Ixodes persulcatus-derived ferritin 2 (IpFER2) showed concentration-dependent iron-binding ability and high amino acid conservation, consistent with FER2s of other tick species. Vaccines containing the recombinant Ip-FER2 elicited a significant reduction of the engorgement weight of adult I. persulcatus. Interestingly, the reduction of engorgement weight was also observed in Ixodes ovatus, a sympatric species of I. persulcatus. In silico analyses of FER2 sequences of I. persulcatus and other ticks showed a greater similarity with I. sccupularis and I. ricinus and lesser similarity with Hyalomma anatolicum, Haemaphysalis longicorras, Rhipicephalus microplus, and R. appendiculatus. Moreover, it was observed that the tick FER2 sequences possess conserved regions within the primary structures, and in silico epitope mapping analysis revealed that antigenic regions were also conserved, particularly among Ixodes spp ticks. In conclusion, the data support further protective tick vaccination applications using the Ip-FER2 antigens identified herein.