Bacteriophage SP6 encodes a second tailspike protein that recognizes Salmonella enterica serogroups C2 and C3
Bacteriophage SP6 encodes a second tailspike protein that recognizes Salmonella enterica serogroups C2 and C3
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DOI：
10.1016/j.virol.2017.02.025
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发表时间：
2017-07-01
期刊：
影响因子：
3.7
通讯作者：
Scholl, Dean
中科院分区：
文献类型：
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作者：
Gebhart, Dana;Williams, Steven R.;Scholl, Dean
SP6 是与大肠杆菌噬菌体 K1-5 密切相关的沙门氏菌噬菌体。 K1-5 值得注意的是，它编码两种多糖降解尾刺蛋白，一种使其能够感染大肠杆菌 K1 的内切唾液酸酶，以及一种使其能够感染 K5 菌株的裂合酶。 SP6 与 K1-5 相似，不同之处在于它编码 P22 样内含鼠李糖苷酶尾刺 (gp46)，使其能够感染 B 组沙门氏菌。我们在此表明，SP6 也可以感染沙门氏菌血清群 C-2 和 C-3，并且假定的第二尾刺 gp47 中的突变消除了这种特异性。将基因47融合至铜绿假单胞菌R2化脓菌素尾纤维的N末端部分的编码区并反式表达，使得融合蛋白掺入化脓菌素颗粒中。这些脓毒素被称为 AvR2-SP47，可杀死血清群 C-2 和 C-3 沙门氏菌。我们得出结论，SP6 编码两种尾部蛋白，使其在肠沙门氏菌中具有广泛的宿主范围。
SP6 is a salmonella phage closely related to coliphage K1-5. K1-5 is notable in that it encodes two polysaccharide-degrading tailspike proteins, an endosialidase that allows it to infect E. coli Kl, and a lyase that enables it to infect K5 strains. SP6 is similar to K1-5 except that it encodes a P22-like endorhamnosidase tailspike, gp46, allowing it to infect group B Salmonella. We show here that SP6 can also infect Salmonella serogroups C-2 and C-3 and that a mutation in a putative second tailspike, gp47, eliminates this specificity. Gene 47 was fused to the coding region of the N-terminal portion of the Pseudomonas aeruginosa R2 pyocin tail fiber and expressed in trans such that the fusion protein becomes incorporated into pyocin particles. These pyocins, termed AvR2-SP47, killed serogroups C-2 and C-3 Salmonella. We conclude that SP6 encodes two tail proteins providing it a broad host range among Salmonella enterica.