Levels of brain-derived neurotrophic factor and neurotrophin-4 in lumbar motoneurons after low-thoracic spinal cord hemisection
Levels of brain-derived neurotrophic factor and neurotrophin-4 in lumbar motoneurons after low-thoracic spinal cord hemisection
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DOI：
10.1016/j.brainres.2004.03.055
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发表时间：
2004-07-09
期刊：
影响因子：
2.9
通讯作者：
Perciavalle, V
中科院分区：
文献类型：
--
作者：
Gulino, R;Lombardo, SA;Perciavalle, V
神经可塑性是脊髓（SC）损伤或传入神经阻滞后的一种常见现象，可补偿脊髓的调制输入损失。神经营养因子在受损脊髓的细胞存活和解剖重组中起着至关重要的作用，并且已知其对神经可塑性发挥活性依赖性调节。很少有人知道他们的作用，在最早的塑料事件，可能涉及突触可塑性，这是负责快速恢复后肢运动后，在大鼠半切。为了获得进一步的了解，我们评估了下胸脊髓半切后腰运动神经元BDNF和NT-4表达的变化。损伤后早期（30分钟），同侧和对侧脊髓腰运动神经元内的免疫染色密度均显著降低。这种减少具有统计学显著性，然后沿着实验期间（14天）显著恢复，在此期间观察到后肢运动的实质性恢复。我们的数据表明，BDNF和NT-4的表达可以通过脊髓回路的活动进行调节，并进一步支持假定的内源性神经营养因子参与脊髓神经可塑性的机制。(C)2004 Elsevier B. V.保留所有权利。
Neuroplasticity represents a common phenomenon after spinal cord (SC) injury or deafferentation that compensates for the loss of modulatory inputs to the cord. Neurotrophins play a crucial role in cell survival and anatomical reorganization of damaged spinal cord, and are known to exert an activity-dependent modulation of neuroplasticity. Little is known about their role in the earliest plastic events, probably involving synaptic plasticity, which are responsible for the rapid recovery of hindlimb motility after hemisection, in the rat. In order to gain further insight, we evaluated the changes in BDNF and NT-4 expression by lumbar motoneurons after low-thoracic spinal cord hemisection. Early after lesion (30 min), the immunostaining density within lumbar motoneurons decreased markedly on both ipsilateral and contralateral sides of the spinal cord. This reduction was statistically significant and was then followed by a significant recovery along the experimental period (14 days), during which a substantial recovery of hindlimb motility was observed. Our data indicate that BDNF and NT-4 expression could be modulated by activity of spinal circuitry and further support putative involvement of the endogenous neurotrophins in mechanisms of spinal neuroplasticity. (C) 2004 Elsevier B.V. All rights reserved.