Survivin gene expression in endometriosis.
Survivin gene expression in endometriosis.
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DOI：
10.1210/jcem.87.7.8682
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发表时间：
2002-07
期刊：
影响因子：
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通讯作者：
M. Ueda;Y. Yamashita;M. Takehara;Y. Terai;K. Kumagai;K. Ueki;K. Kanda;H. Yamaguchi;Daisuke Akise-Da
中科院分区：
文献类型：
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作者：
M. Ueda;Y. Yamashita;M. Takehara;Y. Terai;K. Kumagai;K. Ueki;K. Kanda;H. Yamaguchi;Daisuke Akise-Da
Survivin是一种新型的细胞凋亡抑制因子，在胎儿发育过程中和肿瘤组织中表达，但在正常成人组织和良性疾病中的表达未见报道。我们研究了Survivin基因和蛋白在肿瘤样良性疾病子宫内膜异位症中的表达，并将其与子宫内膜异位症组织的细胞凋亡和侵袭表型相关。从35例子宫内膜异位症患者手术获得的63例色素或非色素增生组织中生存素、基质金属蛋白酶（MMP）-2，MMP-9和膜1型（MT 1）-MMP的基因表达水平与12例非子宫内膜异位症患者的正常在位子宫内膜进行了比较。Survivin、MMP-2、MMP-9和MT 1-MMP mRNA在临床侵袭性色素性子宫内膜病变中的表达均显著高于正常在位内膜，且Survivin在色素性子宫内膜病变中的表达也显著高于非色素性子宫内膜病变（P < 0.05）。Survivin与MMP-2、MMP-9、MT 1-MMP基因表达水平在63例乳腺癌组织中呈显著正相关（P < 0.01）。dUTP缺口末端标记法检测11例卵巢上皮性增生组织中凋亡细胞较少，Survivin和MMPs免疫组化染色阳性。我们的研究结果表明，生存素和MMPs的上调可能协同有助于子宫内膜异位症的生存和侵袭。
Survivin is a novel inhibitor of apoptosis and is expressed during fetal development and in cancer tissues, but its expression has not been reported in normal adult tissues or benign diseases. We investigated survivin gene and protein expression in a tumor-like benign disease, endometriosis, and correlated them with apoptosis and invasive phenotype of endometriotic tissues. Gene expression levels of survivin, matrix metalloproteinase (MMP)-2, MMP-9, and membrane type 1 (MT1)-MMP in 63 pigmented or nonpigmented endometriotic tissues surgically obtained from 35 women with endometriosis were compared with those in normal eutopic endometrium obtained from 12 women without endometriosis. Survivin, MMP-2, MMP-9, and MT1-MMP mRNA expression levels in clinically aggressive pigmented lesions were significantly higher than those in normal eutopic endometrium, and survivin gene expression in pigmented lesions was also higher than that in nonpigmented lesions (P < 0.05). There was a close correlation between survivin and MMP-2, MMP-9, or MT1-MMP gene expression levels in 63 endometriotic tissues examined (P < 0.01). Apoptotic cells detected by the dUTP nick-end labeling were rare in 11 ovarian endometriotic tissues, which showed positive immunohistochemical expression for survivin and MMPs. Our findings suggest that up-regulation of survivin and MMPs may cooperatively contribute to survival and invasion of endometriosis.