Oxygen consumption is a quality marker for human oocyte competence conditioned by ovarian stimulation regimens
Oxygen consumption is a quality marker for human oocyte competence conditioned by ovarian stimulation regimens
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DOI：
10.1016/j.fertnstert.2011.06.059
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发表时间：
2011-09-01
影响因子：
6.7
通讯作者：
Meseguer, Marcos
中科院分区：
文献类型：
--
作者：
Tejera, Aberto;Herrero, Javier;Meseguer, Marcos
目的：评价不同卵巢刺激方案对卵母细胞呼吸的影响，探讨卵母细胞耗氧量与生殖结局的关系。设计：前瞻性观察队列研究。地点：一所大学附属医院的不孕不育诊所。患者：在我们的卵子捐赠计划中，来自56个IVF治疗周期的349个卵母细胞。干预:没有。主要观察指标：平均卵母细胞耗氧量（单位：fmol/s）。我们将耗氧量值与卵巢刺激特征、受精、第2天和第3天胚胎质量以及着床相关联。结果：根据刺激方案中使用的促性腺激素类型的不同，发现测量的耗氧率存在差异。正常受精的卵母细胞消耗率高于未受精或异常卵母细胞消耗率（优势比= 1.340；95%可信区间= 1.037-1.732）。此外，与未植入胚胎的卵母细胞相比，植入胚胎的卵母细胞耗氧量更高（6.21 +/- 0.849 fmol/s vs. 5.23 +/- 0.345 fmol/s）。结论：在受精前测量单个卵母细胞的耗氧率提供了一种无创的卵母细胞质量标记，因此可以定量评估卵母细胞的生殖潜力。[j] .中国农业科学（英文版），2011;26(6):618- 623。(C)2011年美国生殖医学学会。)
Objective: To evaluate the effect of different ovarian stimulation protocols on oocyte respiration and to investigate the relationship between oocyte oxygen consumption and reproductive outcome.Design: Prospective observational cohort study.Setting: Infertility clinic in a university hospital.Patient(s): A total of 349 oocytes from 56 IVF treatment cycles in our oocyte donation program.Intervention(s): None.Main Outcome Measure(s): Average oocyte oxygen consumption rate in fmol/s. We correlated oxygen consumption values with ovarian stimulation features, fertilization, embryo quality on days 2 and 3, and implantation.Result(s): Differences in the measured oxygen consumption rates were found depending on which type of gonadotropins were used in the stimulation protocol. Higher consumption rates were found for oocytes that underwent normal fertilization compared with rates from nonfertilized or abnormal oocytes (odds ratio = 1.340; 95% confidence intervals = 1.037-1.732). Furthermore, higher oxygen consumption was observed for those oocytes which generated embryos that implanted compared with those that did not implant (6.21 +/- 0.849 fmol/s vs. 5.23 +/- 0.345 fmol/s.Conclusion(s): Measurement of oxygen consumption rates for individual oocytes before fertilization provides a noninvasive marker of oocyte quality and hence a quantitative assessment of the reproductive potential for the oocyte. (Fertil Steril (R) 2011;96:618-23. (C)2011 by American Society for Reproductive Medicine.)