SNRPB is a mediator for cellular response to cisplatin in non-small-cell lung cancer
SNRPB is a mediator for cellular response to cisplatin in non-small-cell lung cancer
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DOI：
10.1007/s12032-021-01502-0
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发表时间：
2021-05-01
期刊：
影响因子：
3.4
通讯作者：
Zhang, Longzhen
中科院分区：
文献类型：
--
作者：
Liu, Nianli;Chen, Aoxing;Zhang, Longzhen
小核糖核蛋白多肽B和B ' （SNRPB）是剪接体的核心成分，在mrna前剪接中起关键作用。新出现的证据表明，它与几种癌症的发展有关。我们之前的研究表明SNRPB在非小细胞肺癌（NSCLC）中高表达，并作为癌基因发挥作用。然而，SNRPB是否有助于非小细胞肺癌的顺铂耐药尚不清楚。在本研究中，我们发现SNRPB负调控NSCLC细胞的顺铂耐药。敲除SNRPB可显著降低顺铂诱导的H1299细胞生长抑制、细胞周期阻滞和细胞凋亡。然而，SNRPB在H460细胞中的强制表达可显著促进顺铂诱导的细胞生长抑制、细胞周期阻滞和细胞凋亡。我们的研究结果还表明，SNRPB的过表达增强了顺铂对H460细胞介导的异种移植肿瘤的抑制作用。我们的研究结果表明SNRPB可能是非小细胞肺癌患者对顺铂化疗反应的预测指标。
The small nuclear ribonucleoprotein polypeptides B And B ' (SNRPB) is a core component of spliceosome and plays a key role in pre-mRNA splicing. Emerging evidence suggests that it involves in the development of several types of cancer. Our previous study has demonstrated SNRPB is highly expressed in non-small-cell lung cancer (NSCLC) and functions as an oncogene. However, whether SNRPB contributes to cisplatin resistance in NSCLC is still unknown. In this study, we found that SNRPB negatively regulates cisplatin resistance in NSCLC cells. Knocking out of SNRPB could significantly decrease cisplatin-induced cell growth inhibition, cell cycle arrest and apoptosis in H1299 cells. However, enforced expression of SNRPB in H460 cells can markedly promote cisplatin-induced cell growth inhibition, cell cycle arrest and apoptosis. Our results also indicate that overexpression of SNRPB enhances the inhibitory effects of cisplatin on H460 cell-mediated xenograft tumors. Our results suggest that SNRPB may be a prediction marker for NSCLC patients in response to cisplatin-based chemotherapy.