Chiral ligand exchange capillary electrophoresis using borate anion as a central ion.
Chiral ligand exchange capillary electrophoresis using borate anion as a central ion.
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DOI：
10.1039/b406875k
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发表时间：
2004-11
期刊：
影响因子：
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通讯作者：
S. Kodama;A. Yamamoto;R. Iio;K. Sakamoto;A. Matsunaga;K. Hayakawa
中科院分区：
文献类型：
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作者：
S. Kodama;A. Yamamoto;R. Iio;K. Sakamoto;A. Matsunaga;K. Hayakawa
使用(S)-3-氨基-1,2-丙二醇作为手性选择剂，硼酸根阴离子作为中心离子，通过配体交换毛细管电泳对具有1,3-二醇结构的天然DL-泛酸进行手性拆分。发现 DL-泛酸高分辨率和短迁移时间的最佳条件是 200 mM (S)-3-氨基-1,2-丙二醇和含有 15% 甲醇的 200 mM 硼酸盐缓冲液 (pH 9.2)，在 20°C 下施加 +25 kV 电压，使用 200 nm 直接检测。利用该系统，外消旋泛酸的分辨率(Rs)约为1.7。当使用(S)-1,2-丙二醇、(S)-1,2,3-丙三醇、(S)-1,3-丁二醇或(S)-1-氨基-2-丙醇代替(S)-3-氨基-1,2-丙二醇作为手性配体时，DL-泛酸不发生对映分离。当硼酸盐被 Tris 或硼酸丁酯取代时，没有实现手性分离。因此，三元络合物的氨基和羧基之间的离子相互作用可能在所提出的CE系统对DL-泛酸的对映体分离中发挥重要作用。
Native DL-pantothenic acid, having a 1,3-diol structure, was chirally resolved by ligand exchange capillary electrophoresis using (S)-3-amino-1,2-propanediol as a chiral selector and the borate anion as a central ion. The optimum conditions for both high resolution and short migration time of DL-pantothenic acid were found to be 200 mM (S)-3-amino-1,2-propanediol and 200 mM borate buffer (pH 9.2) containing 15% methanol with an applied voltage of +25 kV at 20 degrees C, using direct detection at 200 nm. With this system, the resolution (Rs) of racemic pantothenic acid was approximately 1.7. When (S)-1,2-propanediol, (S)-1,2,3-propanetriol, (S)-1,3-butanediol or (S)-1-amino-2-propanol were used as chiral ligand instead of (S)-3-amino-1,2-propanediol, DL-pantothenic acid was not enantioseparated. When borate was replaced with Tris or butylborate, no chiral separation was achieved. Therefore, the ionic interaction between the amino and carboxyl groups of the ternary complex may play an important role in the enantioseparation of DL-pantothenic acid by the proposed CE system.