Metabolic DNA as the origin of spontaneously released DNA?
Metabolic DNA as the origin of spontaneously released DNA?
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DOI：
10.1196/annals.1448.046
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发表时间：
2008-01-01
期刊：
影响因子：
--
通讯作者：
Stroun, Maurice
中科院分区：
文献类型：
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作者：
Gahan, Peter B.;Anker, Philippe;Stroun, Maurice
DNA 片段是从活的人类、其他哺乳动物、鸟类、两栖动物、植物和原核细胞中自发释放的，但不是死亡或垂死的细胞。自发释放的 DNA 片段已被证明 (a) 存在于活跃分裂和非分裂、分化的细胞群中； (b) 不稳定； (c)与DNA依赖性RNA或DNA聚合酶相关； (d)与RNA片段相关； (e) 具有比典型遗传 DNA 部分更低的分子量； (f) 与血浆/血清中的独特基因相比，Alu 重复序列的比例增加。另一方面，早期对 DNA 的放射自显影、生物化学以及定量细胞化学和细胞物理研究允许鉴定 DNA 片段，该片段 (1) 存在于活跃分裂和非分裂、分化的细胞群中； (2) 不稳定； (3) 具有比典型遗传 DNA 组分更低的分子量。这种 DNA 片段被称为代谢 DNA (m-DNA)，并被认为可能形成额外的基因拷贝以快速产生 mRNA，随后被破坏。因此，我们认为代谢 DNA 组分可能代表自发释放 DNA 组分形成的前体。
A DNA fraction is spontaneously released from living, but not dead or dying, human, other mammalian, avian, amphibian, plant, and prokaryote cells. The spontaneously released DNA fraction has been shown to be (a) present in both actively dividing and nondividing, differentiated cell populations; (b) labile; (c) associated with DNA-dependent RNA or DNA polymerase; (d) associated with an RNA fraction; and to have (e) a lower molecular weight than the typical genetic DNA fraction; and (f) Alu repeat sequences in increased proportions compared to a unique gene in plasma/serum. On the other hand, early autoradiographic and biochemical and quantitative cytochemical and cytophysical studies on DNA permitted the identification of a DNA fraction which was (1) present in both actively dividing and nondividing, differentiated cell populations; (2) labile; and (3) had a lower molecular weight than the typical genetic DNA fraction. This DNA fraction was termed metabolic DNA (m-DNA) and was proposed as possibly forming extra gene copies for the rapid production of m-RNA, to be destroyed subsequently. Therefore, we suggest that the metabolic DNA fraction might represent the precursor to the formation of the spontaneously released DNA fraction.