Folate-bovine serum albumin functionalized polymeric micelles loaded with superparamagnetic iron oxide nanoparticles for tumor targeting and magnetic resonance imaging
Folate-bovine serum albumin functionalized polymeric micelles loaded with superparamagnetic iron oxide nanoparticles for tumor targeting and magnetic resonance imaging
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叶酸-牛血清白蛋白功能化聚合物胶束负载超顺磁性氧化铁纳米颗粒用于肿瘤靶向和磁共振成像
DOI：
10.1016/j.actbio.2015.01.006
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发表时间：
2015-03-15
影响因子：
9.7
通讯作者：
Chu, Paul K.
中科院分区：
文献类型：
--
作者：
Li, Huan;Yan, Kai;Chu, Paul K.
研究了叶酸偶联牛血清白蛋白(FA-BSA)修饰的超顺磁性氧化铁纳米粒子(SPIOns)聚合物胶束作为肿瘤靶向和体内核磁共振成像(MRI)的特异性造影剂。通过油酸修饰的Fe3O4纳米粒子与两亲性聚(HFMA-co-MOTAC)-g-PEGMA共聚物的自组装制备了负载SPIONS的聚合物胶束，并通过静电作用使其与FA-BSA功能化。制备的FA-BSA改性磁性胶束的流体力学直径为196.1 nm，饱和磁化强度为5.5emu/g，横向弛豫系数为167.0 mm(-1)S。体外磁共振成像、普鲁士蓝染色和细胞内铁测定研究表明，叶酸功能化的磁性胶束对叶酸受体阳性的肝癌细胞Bel-7402具有更大的细胞摄取能力。静脉推注给药后，在荷瘤Bel-7402裸鼠身上进行的体内磁共振成像显示出良好的肿瘤靶向性和磁共振成像能力，尤其是在注射后24小时。这些发现表明，FA-BSA修饰的磁性胶束在肿瘤检测中具有作为靶向MRI探针的潜力。(C)2015 Acta Materialia Inc.由爱思唯尔有限公司出版。版权所有。
Polymeric micelles functionalized with folate conjugated bovine serum albumin (FA-BSA) and loaded with superparamagnetic iron oxide nanoparticles (SPIONs) are investigated as a specific contrast agent for tumor targeting and magnetic resonance imaging (MRI) in vitro and in vivo. The SPIONs-loaded polymeric micelles are produced by self-assembly of amphiphilic poly(HFMA-co-MOTAC)-g-PEGMA copolymers and oleic acid modified Fe3O4 nanoparticles and functionalized with FA-BSA by electrostatic interaction. The FA-BSA modified magnetic micelles have a hydrodynamic diameter of 196.1 nm, saturation magnetization of 5.5 emu/g, and transverse relaxivity of 167.0 mM(-1) S-1. In vitro MR imaging, Prussian blue staining, and intracellular iron determination studies demonstrate that the folate-functionalized magnetic micelles have larger cellular uptake against the folate-receptor positive hepatoma cells Bel-7402 than the unmodified magnetic micelles. In vivo MR imaging conducted on nude mice bearing the Bel-7402 xenografts after bolus intravenous administration reveals excellent tumor targeting and MR imaging capabilities, especially at 24 h post-injection. These findings suggest the potential of FA-BSA modified magnetic micelles as targeting MRI probe in tumor detection. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.