3D reconstruction of the hemocyanin subunit dimer from the chiton Acanthochiton fascicularis
3D reconstruction of the hemocyanin subunit dimer from the chiton Acanthochiton fascicularis
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DOI：
10.1016/j.micron.2003.10.008
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发表时间：
2004-01-01
期刊：
影响因子：
2.4
通讯作者：
Markl, J
中科院分区：
文献类型：
--
作者：
Harris, JR;Meissner, U;Markl, J
介绍了从纤毛棘皮血蓝蛋白中分离纯化亚单位二聚体的方法。阴性染色标本的电子显微镜显示，具有典型的“船形”特征的大分子均匀分布。根据这一EM数据的3D重建产生了类似于3 nm分辨率的模型，该模型与据称的亚单位二聚体的早期数据很好地相关，该亚单位二聚体是从鲍鱼血蓝蛋白类型1的十二聚体的3D重建中提取的。(C)2003 Elsevier Ltd.保留所有权利。
Procedures are presented for the purification of the subunit dimer from Acanthochiton fasicularis hemocyanin. Electron microscopy of negatively stained specimens revealed a uniform population of macromolecules possessing the characteristic "boat shape". A 3D reconstruction from this EM data generated a similar to 3 nm resolution model that correlates well with earlier data of the purported subunit dimer, extracted from the 3D reconstruction of the didecamer of Haliotis tuberculata hemocyanin type 1. (C) 2003 Elsevier Ltd. All rights reserved.