The distribution of lagomorph syphilis caused by Treponema paraluisleporidarum in Europe
The distribution of lagomorph syphilis caused by Treponema paraluisleporidarum in Europe
复制标题
DOI：
10.1007/s10344-021-01535-w
复制
发表时间：
2021-10-01
影响因子：
2
通讯作者：
Knauf, Sascha
中科院分区：
文献类型：
--
作者：
Hisgen, Linda;Abel, Lena;Knauf, Sascha
副孢子密螺旋体既可感染兔(兔)，也可感染兔(兔)。虽然此前在欧洲褐兔(Lepus Europaeus)和家兔(Oryctolagus cunrulus F.home tica)中曾报告过这种细菌的发生，但目前还没有数据报告在欧洲范围内感染。我们从随机抽样的欧洲褐兔(Lepus Europaeus；n=2135)、山兔(Lepus timidus；n=4)、欧洲兔(Oryctolagus ccunrulus；n=138)和宠物兔(O.cctolagus F.home tica；n=5)总共检测了1,995份血清样本和287份生殖器拭子。这些样品来自八个欧洲国家。在只有血清可用的情况下，我们对样本进行了抗密螺旋体抗体的检测。为此，我们采用梅毒螺旋体颗粒凝集试验(TP-PA)，由于抗梅毒螺旋体抗体的抗原交叉反应，该试验适用于兔形体。梅毒和抗T。副孢子虫抗体。另外，用荧光密螺旋体抗体吸收试验(FTA-ABS)对380份血清的结果进行了确认。在所有有拭子样本的情况下，提取DNA并使用定量PCR进行检测，以检测拉格莫夫梅毒细菌的存在。我们在1,995份兔血清中检测到825份抗体(41.4%；棕兔：825/1,868；兔：0/127)，并在287份拭子样本中有182份获得了qPCR阳性结果(63.4%；欧洲褐兔：167/267；山兔：4/4；兔：11/16)。所有兔血清(n=127)抗梅毒螺旋体抗体检测均为阴性，8只野兔(n=8/11)和3只德国家兔(n=3/5)经qPCR检测证实存在梅毒螺旋体。
Treponema paraluisleporidarum infects both rabbits (ecovar Cuniculus) and hares (ecovar Lepus). While the occurrence of the bacterium has previously been reported for European brown hares (Lepus europaeus) and domestic rabbits (Oryctolagus cuniculus f. domestica), there are no data available that report infection in the European context. We tested a total of 1,995 serum samples and 287 genital swabs from opportunistically sampled European brown hares (Lepus europaeus; n = 2135), Mountain hares (Lepus timidus; n = 4), European rabbits (Oryctolagus cuniculus; n = 138), and pet rabbits (O. cuniculus f. domestica; n = 5). The samples originated from eight European countries. In case only serum was available, we tested the samples for the presence of anti-treponemal antibodies. For this, we utilized the Treponema pallidum-particle agglutination test (TP-PA), which is suited for the use in lagomorphs due to the antigenic cross-reactivity of anti-T. pallidum and anti-T. paraluisleporidarum antibodies. In addition, the results of 380 sera were confirmed using the fluorescent-Treponema antibody absorption test (FTA-ABS). In all cases where swab samples were available, DNA was extracted and tested using quantitative PCR to test for the presence of the lagomorph syphilis-bacterium. We were able to detect antibodies in 825 of 1,995 lagomorph sera (41.4%; brown hare: 825/1,868; rabbit: 0/127) and obtained positive qPCR results from 182 of 287 swab samples (63.4%; European brown hare: 167/267; mountain hare: 4/4; rabbit: 11/16). While all rabbit sera (n = 127) tested negative for anti-treponemal antibodies, the presence of the bacterium was confirmed in eight wild (n = 8/11) and three domestic rabbits (n = 3/5) from Germany using qPCR.