Quercetin Attenuates Oxidative Damage Induced by Treatment of Embryonic Chicken Spermatogonial Cells with 4-Nitro-3-phenylphenol in Diesel Exhaust Particles
Quercetin Attenuates Oxidative Damage Induced by Treatment of Embryonic Chicken Spermatogonial Cells with 4-Nitro-3-phenylphenol in Diesel Exhaust Particles
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槲皮素可减轻柴油机尾气颗粒中的 4-硝基-3-苯基苯酚处理鸡胚精原细胞引起的氧化损伤
DOI：
10.1271/bbb.90740
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发表时间：
2010-05-01
影响因子：
1.6
通讯作者：
Taya, Kazuyoshi
中科院分区：
文献类型：
--
作者：
Mi, Yuling;Zhang, Caiqiao;Taya, Kazuyoshi
槲皮素是一种抗氧化剂类黄酮，被认为对人类和动物健康有益。在这项研究中，槲皮素的保护作用，睾丸细胞的氧化损伤后，在鸡胚处理的4-硝基-3-苯基苯酚（PNMPP）来自柴油机尾气颗粒物。用PNMPP（10−8-10−6 M）单独和与槲皮素联合攻击睾丸细胞48 h。结果表明，槲皮素浓度在1.0 μg/ml时对精原细胞无明显毒性作用。暴露于PNMPP（10−6 M）可诱导细胞核浓缩和细胞质空泡化，睾丸细胞活力和精原细胞数量减少（p<0.05）。硫代巴比妥酸反应物升高，谷胱甘肽过氧化物酶活性和超氧化物歧化酶活性降低（P<0.05），引起脂质过氧化反应。同时补充槲皮素将这些参数恢复到与对照组相同的水平。这些数据表明，槲皮素保护PNMPP中毒的鸡胚精原细胞免受氧化损伤。
Quercetin, an antioxidant flavonoid, is considered beneficial for human and animal health. In this study, the protective effect of quercetin on oxidative damage to testicular cells was studied in embryonic chickens after treatment with 4-nitro-3-phenylphenol (PNMPP) derived from diesel exhaust particles. Testicular cells were challenged with PNMPP (10−8–10−6 M) alone and in combination with quercetin for 48 h. The results showed that quercetin manifested no deleterious effect on spermatogonial cells up to 1.0 μg/ml. Exposure to PNMPP (10−6 M) induced condensed nuclei and vacuolated cytoplasm and reductions in testicular cell viability and spermatogonial cell numbers (p<0.05). It also induced lipid peroxidation by an elevation of thiobarbituric acid reactive substances and decreased glutathione peroxidase activity and superoxide dismutase activity (p<0.05). Simultaneous supplementation with quercetin restored these parameters to the same levels as in the control. These data indicate that quercetin protects spermatogonial cells from oxidative damage in embryonic chickens intoxicated with PNMPP.