53BP2 induces apoptosis through the mitochondrial death pathway
53BP2 induces apoptosis through the mitochondrial death pathway
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DOI：
10.1111/j.1365-2443.2005.00835.x
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发表时间：
2005-03-01
期刊：
影响因子：
2.1
通讯作者：
Okamoto, T
中科院分区：
文献类型：
--
作者：
Kobayashi, S;Kajino, S;Okamoto, T
p53结合蛋白2（53 BP 2）是与p53、Bcl-2和核因子κ B亚基（NF-κ B）p65相互作用的蛋白。TP 53 BP 2基因编码两种剪接变体，53 BP 2S和53 BP 2L，以前称为p53的凋亡刺激蛋白2（ASPP 2）。我们发现，这些53 BP 2蛋白主要位于细胞质中，并诱导细胞凋亡，如聚ADP核糖聚合酶（PARP）和膜联蛋白V染色的切割所示。此外，我们证明，53 BP 2是位于线粒体和诱导凋亡与抑郁症的线粒体跨膜电位（DeltaPsim）和激活caspase-9。从这些发现中，我们得出结论，53 BP 2通过线粒体死亡途径诱导细胞凋亡。
The p53 binding protein 2 (53BP2) has been identified as the interacting protein to p53, Bcl-2, and p65 subunit of nuclear factor kappaB (NF-kappaB). The TP53BP2 gene encodes two splicing variants, 53BP2S and 53BP2L, previously known as apoptosis stimulating protein 2 of p53 (ASPP2). We found that these 53BP2 proteins are located predominantly in the cytoplasm and induce apoptosis as demonstrated by cleavage of poly ADP ribose polymerase (PARP) and annexin V staining. Furthermore, we demonstrate that 53BP2 is located in the mitochondria and induces apoptosis associated with depression of the mitochondrial trans-membrane potential (DeltaPsim) and activation of caspase-9. From these findings we conclude that 53BP2 induces apoptosis through the mitochondrial death pathway.