Inoculum size and intraspecific interactions affects Penicillium expansum growth and patulin accumulation in apples.
Inoculum size and intraspecific interactions affects Penicillium expansum growth and patulin accumulation in apples.
复制标题
DOI：
10.1016/j.fm.2007.09.008
复制
发表时间：
2008-02
影响因子：
5.3
通讯作者：
H. Morales;V. Sanchis;J. Coromines;A. Ramos;S. Marín
中科院分区：
文献类型：
--
作者：
H. Morales;V. Sanchis;J. Coromines;A. Ramos;S. Marín
扩展青霉（Penicilliumexpansum）是引起冷藏苹果腐败变质的重要真菌。扩展青霉产生展青霉素，一种有毒的次级代谢物。本工作的目的是研究生长参数的变化和展青霉素积累时，可能会发生两个不同的分离物的扩展青霉在同一伤口发芽和发展。还研究了接种量的影响。为此目的，用两种不同分生孢子浓度的扩展疫霉的两种不同分离物点接种苹果。两种分离株也一起接种。将苹果冷藏在1°C下或在20°C下孵育，直到病变直径达到2.5cm。在冷藏/孵育结束时分析来自腐烂组织的棒曲霉素。似乎种内的相互作用发生。相互作用导致生长较慢，其中棒曲霉素含量较低。这可能涉及对资源的竞争，随后是“类似战斗”的互动。较高的接种量导致较短的滞后期，这反映了包装车间设施卫生的重要性。接种量也影响生长速率和展青霉素积累，这表明同一菌株的个体之间可能发生相互作用。可能会发生资源竞争和类似战斗的互动。应进行体外研究，以评估观察到的相互作用模式。
Penicillium expansum is the most important fungal species causing spoilage in cold stored apples. P. expansum produces patulin, a toxic secondary metabolite. The aim of this work was to study the changes in growth parameters and patulin accumulation that may occur when two different isolates of P. expansum germinate and develop in the same wound. The effect of the inoculum size was also studied. For that purpose, apples were point inoculated with two different isolates of P. expansum at two different conidia concentrations. Both isolates were also inoculated together. Apples were cold stored at 1°C or incubated at 20°C until lesion diameter reached 2.5cm. Patulin from decayed tissue was analysed at the end of cold storage/incubation. It seems that intraspecific interactions occur. The interactions resulted in slower growth in which patulin content was lower. A competition for resources followed by either a “combat-like” interaction may be involved in this. Higher inoculum size led to shorter lag phase, which reflects the importance of the hygiene in packinghouses facilities. The inoculum size also affected growth rate and patulin accumulation, which suggests that interactions between individuals of the same strain may occur. Competition for resources and a combat-like interaction may take place. In vitro studies should be carried out in order to assess the patterns of the interactions observed.