Studies of the UVC-sensitivity of non-tumorigenic and tumorigenic human cell hybrids (HeLa x skin fibroblasts).
Studies of the UVC-sensitivity of non-tumorigenic and tumorigenic human cell hybrids (HeLa x skin fibroblasts).
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非致瘤性和致瘤性人类细胞杂交体（HeLa x 皮肤成纤维细胞）的 UVC 敏感性研究。
DOI：
10.1080/09553008914551051
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发表时间：
1989
影响因子：
2.6
通讯作者：
Sun,C
中科院分区：
文献类型：
--
作者：
Redpath,JL;Antoniono,R;Sun,C
比较和对比非致瘤性和致瘤性人类细胞杂交体（HeLa × 皮肤成纤维细胞）的 UVC 敏感性。致瘤细胞与非致瘤细胞的不同之处在于，它们丢失了 11 号和 14 号染色体各一份。对于指数生长的培养物，致瘤细胞比非致瘤细胞具有显着更强的抵抗力。对于汇合培养物，光敏性的差异要小得多。对这些细胞的指数生长和汇合培养物的细胞周期分布的流式细胞术研究表明，光敏性的差异不能用细胞周期分布的差异来解释。对两种细胞系汇合培养物中潜在致命损伤修复 (PLDR) 动力学的研究表明，在前 6 小时内几乎没有或没有恢复，随后在接下来的 12-24 小时内存活率增加。这些数据与之前发表的人类皮肤成纤维细胞的观察结果一致，其中 PLDR 的动力学反映了胸腺嘧啶二聚体损失的动力学。这些数据与 6-4 光产物是潜在致命损伤的说法不一致，因为这种损伤在人体细胞中可以迅速修复。
The UVC-sensitivities of a non-tumorigenic and a tumorigenic human cell hybrid (HeLa × skin fibroblasts) are compared and contrasted. The tumorigenic cells differ from the non-tumorigenic cells in that they have lost one copy each of chromosomes 11 and 14. For exponentially growing cultures, the tumorigenic cells are considerably more resistant than the non-tumorigenic cells. For confluent cultures, the differential in photosensitivity is much less. Flow cytometric studies of cell cycle distributions of both exponentially growing and confluent cultures of these cells indicate that the differences in photosensitivity cannot be explained by differences in cell cycle distribution. Studies of the kinetics of potentially lethal damage repair (PLDR) in confluent cultures of both cell lines indicate little or no recovery over the first 6 h followed by an increase in survival over the next 12–24 h. These data are consistent with previously published observations in human skin fibroblasts where the kinetics of PLDR reflected the kinetics of thymine dimer loss. The data are not consistent with 6–4 photoproducts being a potentially lethal lesion since such damage is rapidly repaired in human cells.