Layer-controlled hemoglobin vesicles by interaction of hemoglobin with a phospholipid assembly
Layer-controlled hemoglobin vesicles by interaction of hemoglobin with a phospholipid assembly
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通过血红蛋白与磷脂组装体的相互作用实现层控制的血红蛋白囊泡
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
E. Tsuchida
中科院分区：
文献类型：
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作者：
S. Takeoka;T. Ohgushi;K. Terase;T. Ohmori;E. Tsuchida
在各种制备条件下制备血红蛋白囊泡，其用二棕榈酰磷脂酰胆碱/胆固醇/棕榈酸的双层包封浓缩血红蛋白（Hb），以减少构成囊泡的双层的数目（n）并增加囊泡内部的Hb浓度（[Hb] in）。当Hb的表面电位由正变负时，[Hb] in由于Hb与双层膜表面间的静电斥力而急剧下降。温度降低导致Hb囊泡（[Hb]/[脂质]）的质量增加，尽管[Hb]由于Hb溶液的粘度增加而降低。这是由于膜流动性的降低和Hb的质子化而导致的n的有效降低。
Hemoglobin vesicles, which encapsulated concentrated hemoglobin (Hb) with a bilayer of dipalmitoylphosphatidylcholine/cholesterol/palmitic acid, were prepared under various preparation conditions in order to decrease the number of bilayers (n) constructing the vesicle and increase the Hb concentration in the interior of the vesicle ([Hb] in ). n decreased when the surface potential of the bilayer became more negative because of the electrostatic repulsion between the bilayers, while with a changing ζ-potential of Hb from positive to negative, [Hb] in showed a precipitous fall because of the electrostatic repulsion between Hb and the surface of the bilayer. A temperature decrease leads to a quality increase in the Hb vesicles ([Hb]/[lipid]) in spite of the [Hb] in decrease by the viscosity increase of the Hb solution. This is explained by the effective reduction of n due to the reduction in membrane fluidity and the protonation of Hb.