Expression of heat shock protein 47, transforming growth factor-beta 1, and connective tissue growth factor in liver tissue of patients with Schistosoma japonicum-induced hepatic fibrosis
Expression of heat shock protein 47, transforming growth factor-beta 1, and connective tissue growth factor in liver tissue of patients with Schistosoma japonicum-induced hepatic fibrosis
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日本血吸虫性肝纤维化患者肝组织中热休克蛋白47、转化生长因子-β1、结缔组织生长因子的表达
DOI：
10.1017/s0031182014001115
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发表时间：
2015-02-01
期刊：
影响因子：
2.4
通讯作者：
Ning, Qin
中科院分区：
文献类型：
--
作者：
Li, Lan;Wu, Ting;Ning, Qin
检测促纤维化分子如热休克蛋白47（Hsp 47）、转化生长因子β 1（TGF-β 1）的表达检测42例慢性血吸虫病（CS）患者肝组织中TGF-β_1、CTGF的表达，分析其与肝纤维化进展的关系。16例慢性B型肝炎（CH B）患者和5例健康人（HI）。免疫组化（IHC）分析显示，与HI组相比，CS组和CHB组Hsp 47、TGF-β 1和CTGF的表达均增加。实时荧光定量PCR检测结果显示，CS患者Hsp 47、TGF-β 1和CTGF的mRNA水平均高于HI患者。在CS患者中，这些基因的mRNA水平与纤维化阶段相关，TGF-β 1 mRNA表达与炎症分级相关。进一步的分析表明，Hsp 47和CTGF的mRNA水平与肝硬度值和脾厚度直径高度相关，这两者都代表了纤维化的严重程度。结论：这三种分子参与了日本血吸虫感染肝纤维化的发病过程。TGF-β 1不仅参与炎症过程，而且参与纤维化过程，其中Hsp 47和CTGF可能起关键作用。
To detect the expression of pro-fibrotic molecules, such as heat shock protein 47 (Hsp47), transforming growth factor-beta 1 (TGF-beta 1) and connective tissue growth factor (CTGF) in liver specimens, and analyse their correlations with the progression of schistosomal hepatic fibrosis, liver biopsy was performed in 42 chronic schistosomiasis (CS) patients, 16 chronic hepatitis B (CHB) patients and five healthy individuals (HI). Immunohistochemistry (IHC) analyses displayed that the expression of Hsp47, TGF-beta 1 and CTGF was increased in CS and CHB patients compared with HI. Using real-time PCR, the mRNA levels of Hsp47, TGF-beta 1 and CTGF were higher in CS patients compared with HI. In CS patients, the mRNA levels of these genes were correlated with the stage of fibrosis, and TGF-beta 1 mRNA expression was associated with the grade of inflammation. Additional analyses indicated that the mRNA levels of Hsp47 and CTGF were highly correlated with liver stiffness value and spleen thickness diameter, both of which represented the severity of fibrosis. In conclusion, the three molecules are involved in the pathogenesis of hepatic fibrosis infected by Schistosoma japonicum. TGF-beta 1 participates not only in the inflammatory process, but also in the fibrotic process in which Hsp47 and CTGF probably play a key role.