Integrating Association Mapping, Linkage Mapping, Fine Mapping with RNA Seq Conferring Seedling Vigor Improvement for Successful Crop Establishment in Deep Sown Direct-Seeded Rice.
Integrating Association Mapping, Linkage Mapping, Fine Mapping with RNA Seq Conferring Seedling Vigor Improvement for Successful Crop Establishment in Deep Sown Direct-Seeded Rice.
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DOI：
10.1186/s12284-023-00665-w
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发表时间：
2023-10-17
期刊：
影响因子：
5.5
通讯作者：
Kumar, Arvind
中科院分区：
文献类型：
--
作者：
Sandhu, Nitika;Ankush, Ade Pooja;Singh, Jasneet;Raigar, Om Prakash;Bains, Sutej;Jindal, Taveena;Singh, Mohini Prabha;Sethi, Mehak;Pruthi, Gomsie;Augustine, Gaurav;Verma, Vikas Kumar;Goyal, Shivani;Kumar, Aman;Panwar, Harsh;Sihag, Manvesh Kumar;Kaur, Rupinder;Kurup, Smita;Kumar, Arvind
目前正在大规模转向直播水稻（DSR），这需要将育种和遗传方法结合起来，以维持其生存并利用自然资源和环境效益。提高幼苗活力仍然是育种者使用DSR的关键目标。本研究的目的是了解深播DSR幼苗活力的遗传控制。本研究采用全基因组关联作图、连锁作图、精细作图、RNA测序等方法对候选基因进行了鉴定和验证。在F3：4：5和BC 2F 2：3群体中，基因型间均存在显著的表型变异。中胚轴长度与发芽率、根长和芽长呈极显著正相关。据报道，第7号染色体上的881 kb区域与中胚轴伸长有关。RNA-seq数据和RT-PCR结果鉴定并验证了7个潜在的候选基因。筛选出4个无连锁阻力、中胚轴长、根长、半矮秆的渐渗系。本研究将为水稻育种工作者提供（1）具有在深播DSR田间条件下提高发芽率的有用性状和等位基因的预期DSR适应渐渗系的预育种材料（2）候选基因功能鉴定的研究基础。改良导入系和分子标记的开发和利用将在基因组辅助育种（GAB）中发挥重要作用。我们的研究结果提供了一个强大的和可靠的包，可以有助于提高直播水稻育种计划的遗传增益。在线版本包含补充材料，可通过10.1186/s12284-023-00665-w获得。
Ongoing large-scale shift towards direct seeded rice (DSR) necessitates a convergence of breeding and genetic approaches for its sustenance and harnessing natural resources and environmental benefits. Improving seedling vigour remains key objective for breeders working with DSR. The present study aims to understand the genetic control of seedling vigour in deep sown DSR. Combined genome-wide association mapping, linkage mapping, fine mapping, RNA-sequencing to identify candidate genes and validation of putative candidate genes were performed in the present study. Significant phenotypic variations were observed among genotypes in both F3:4:5 and BC2F2:3 populations. The mesocotyl length showed significant positive correlation with %germination, root and shoot length. The 881 kb region on chromosome 7 reported to be associated with mesocotyl elongation. RNA-seq data and RT-PCR results identified and validated seven potential candidate genes. The four promising introgression lines free from linkage drag and with longer mesocotyl length, longer root length, semi-dwarf plant height have been identified. The study will provide rice breeders (1) the pre breeding material in the form of anticipated DSR adapted introgression lines possessing useful traits and alleles improving germination under deep sown DSR field conditions (2) the base for the studies involving functional characterization of candidate genes. The development and utilization of improved introgression lines and molecular markers may play an important role in genomics-assisted breeding (GAB) during the pyramiding of valuable genes providing adaptation to rice under DSR. Our results offer a robust and reliable package that can contribute towards enhancing genetic gains in direct seeded rice breeding programs. The online version contains supplementary material available at 10.1186/s12284-023-00665-w.
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影响因子：
7.2
作者：
Daniel, X;Lacomme, C;Roby, D
通讯作者：
Roby, D
DOI：
10.1073/pnas.0805303105
发表时间：
2008-08-26
影响因子：
11.1
作者：
Fujino, Kenji;Sekiguchi, Hiroshi;Yano, Masahiro
通讯作者：
Yano, Masahiro
影响因子：
5.4
作者：
Mahuku, George;Chen, Jiafa;Xu, Yunbi
通讯作者：
Xu, Yunbi
影响因子：
--
作者：
REGAN, KL;SIDDIQUE, KHM;WHAN, BR
通讯作者：
WHAN, BR
影响因子：
6.6
作者：
Meng, Lei;Li, Huihui;Wang, Jiankang
通讯作者：
Wang, Jiankang