Effects of neuropeptide Y on sucrose and ethanol intake and on anxiety-like behavior in high alcohol drinking (HAD) and low alcohol drinking (LAD) rats
Effects of neuropeptide Y on sucrose and ethanol intake and on anxiety-like behavior in high alcohol drinking (HAD) and low alcohol drinking (LAD) rats
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DOI：
10.1111/j.1530-0277.2003.tb04413.x
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发表时间：
2003-06-01
影响因子：
3.2
通讯作者：
Li, TK
中科院分区：
文献类型：
--
作者：
Badia-Elder, NE;Stewart, RB;Li, TK
背景资料：在以前的研究中，神经肽Y（NPY）的管理到侧脑室减少酒精偏好（P）大鼠的乙醇摄入量，但在酒精非偏好（NP）或非酒精性Wistar大鼠。本调查的目的是延长这些研究结果在选择性繁殖的高酒精饮用（HAD）1和低酒精饮用（LAD）1大鼠通过检查的影响，脑室内注射神经肽Y的高架十字迷宫测试的焦虑和对乙醇和蔗糖intake.Methods：女性HAD 1和LAD 1大鼠手术植入套管到侧脑室。恢复后，进行焦虑测试，其中大鼠（n = 12-13/组）在高架十字迷宫上进行5分钟测试之前10分钟接受人工脑脊液（aCSF）或NPY（10 μ g）。焦虑测试后，11只HAD和11只LAD大鼠接受自我给药乙醇（8% w/v）的训练，5只HAD和8只LAD大鼠接受自我给药蔗糖（2.5%）的训练，每日2小时。采用受试者内设计，在饮酒前每周用aCSF、5 μ g NPY或10 μ g NPY预处理大鼠一次。结果：用aCSF治疗的HAD和LAD大鼠在开放臂中花费的时间没有差异十字迷宫。在两种大鼠系中，NPY增加了在开放臂上花费的时间，其程度相似。HAD大鼠消耗更多的乙醇和蔗糖比LAD大鼠。NPY增加蔗糖摄入量在这两个大鼠线。然而，相同剂量的神经肽Y减少酒精摄入量在HAD，但不是在LAD rats.Conclusion：加迷宫结果表明，选择性繁殖高和低酒精偏好的HAD 1和LAD 1大鼠，分别没有产生差异的焦虑样行为和响应的抗焦虑作用的神经肽Y。蔗糖摄入量的增加与已知的神经肽Y的促食欲作用一致。HAD大鼠NPY给药后乙醇摄入量减少与P大鼠的先前观察结果相似，并且与乙醇摄入量和NPY活性可能呈负相关的假设一致。
Background: In a previous study, neuropeptide Y (NPY) administered into the lateral ventricles decreased ethanol intake in alcohol-preferring (P) rats but not in alcohol-nonpreferring (NP) or unselected Wistar rats. The purpose of the present investigation is to extend these findings in selectively-bred high-alcohol-drinking (HAD)1 and low-alcohol-drinking (LAD)1 rats by examining the effects of intracerebroventricularly administered NPY on the elevated plus maze test of anxiety and on ethanol and sucrose intake.Methods: Female HAD1 and LAD1 rats were surgically implanted with cannula into the lateral ventricle. Following recovery, a test of anxiety was conducted in which the rats (n = 12-13/group) received either artificial cerebrospinal fluid (aCSF) or NPY (10 mug) 10 min prior to a 5-min test on an elevated plus maze. Following anxiety testing, 11 HAD and 11 LAD rats were trained to self-administer ethanol (8% w/v), and 5 HAD and 8 LAD rats were trained to self-administer sucrose (2.5%) during daily 2-hr sessions. A within-subject design was used in which the rats were pretreated once a week with aCSF, 5 mug NPY, or 10 mug NPY prior to the drinking sessions.Results: HAD and LAD rats treated with aCSF did not differ in time spent in open arms of the plus maze. NPY increased time spent on the open arms to similar degrees in both rat lines. HAD rats consumed more ethanol and sucrose than LAD rats. NPY increased sucrose intake in both rat lines. However, the same doses of NPY reduced ethanol intake in HAD but not in LAD rats.Conclusion: The plus maze results indicated that selective breeding for high and low alcohol preference in the HAD1 and LAD1 rats, respectively, did not yield differences in anxiety-like behavior and in response to the anxiolytic effects of NPY. The increases in sucrose intake were consistent with the known orexigenic effects of NPY. The decreased ethanol intake following NPY administration in HAD rats was similar to previous observations with P rats and is consistent with the hypothesis that ethanol intake and NPY activity may be inversely related.